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Protective Effect of HO-1 Transfection against Ethanol-induced Osteoblast Damage 被引量:2

Protective Effect of HO-1 Transfection against Ethanol-induced Osteoblast Damage
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摘要 Heme oxygenase-1(HO-1) plays important roles in anti-oxidant, anti-inflammatory and immunoregulative activities. The aim of this study was to observe if HO-1 transfection could inhibit the damage of osteoblasts induced by ethanol. HO-1 was transfected into osteoblasts via constructed plasmid. After exposure to ethanol for 24 h, cytoactivity and apoptosis of osteoblasts were measured by MTT assay and flow cytometry, respectively. Furthermore, the oxidative stress and inflammatory factors in osteoblasts were measured. Compared to positive control group, the cytoactivity of transfected osteoblasts was significantly increased, and the apoptosis rate was significantly decreased(P〈0.05). At the same time, the levels of reactive oxygen species(ROS), methane dicarboxylic aldehyde(MDA), tumor necrosis factor-α(TNF-α) and interleukin-1(IL-1) were significantly decreased(P〈0.05), and superoxide dismutase(SOD) level was increased(P〈0.05) in the transfected osteoblasts as compared with positive controls. These results suggest that HO-1 plays a protective role in osteoblasts, and HO-1 transfection can effectively inhibit bone damage induced by ethanol. Heme oxygenase-1(HO-1) plays important roles in anti-oxidant, anti-inflammatory and immunoregulative activities. The aim of this study was to observe if HO-1 transfection could inhibit the damage of osteoblasts induced by ethanol. HO-1 was transfected into osteoblasts via constructed plasmid. After exposure to ethanol for 24 h, cytoactivity and apoptosis of osteoblasts were measured by MTT assay and flow cytometry, respectively. Furthermore, the oxidative stress and inflammatory factors in osteoblasts were measured. Compared to positive control group, the cytoactivity of transfected osteoblasts was significantly increased, and the apoptosis rate was significantly decreased(P〈0.05). At the same time, the levels of reactive oxygen species(ROS), methane dicarboxylic aldehyde(MDA), tumor necrosis factor-α(TNF-α) and interleukin-1(IL-1) were significantly decreased(P〈0.05), and superoxide dismutase(SOD) level was increased(P〈0.05) in the transfected osteoblasts as compared with positive controls. These results suggest that HO-1 plays a protective role in osteoblasts, and HO-1 transfection can effectively inhibit bone damage induced by ethanol.
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第3期374-377,共4页 华中科技大学学报(医学英德文版)
关键词 HO-1 ETHANOL OSTEOBLAST apoptosis TNF-α IL-1 reactive oxygen species methane dicarboxylic aldehyde superoxide dismutase HO-1 ethanol osteoblast apoptosis TNF-α IL-1 reactive oxygen species methane dicarboxylic aldehyde superoxide dismutase
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