比较 Bruker Microflex MALDI-TOF MS和 Vitek 2 Compact 全自动分析系统对酵母菌的鉴定能力

Comparison of yeast identification ability between Bruker Microflex MALDI-TOF MS and Vitek 2 Compact automatic microbial analysis system

目的：比较Bruker Microflex MALDI-TOF MS和Vitek 2 Compact全自动微生物分析系统对酵母菌的鉴定能力。方法回顾性研究。利用 Bruker Microflex MALDI-TOF MS 和 Vitek 2 Compact全自动微生物分析系统同时对2013年3月至2014年3月上海交通大学医学院附属新华医院临床标本分离得到的742株酵母菌进行鉴定，结果不符菌株用基因测序予以确证。结果 Bruker Microflex MALDI-TOF MS和Vitek 2 Compact全自动微生物分析系统对699株念珠菌的种鉴定符合率分别为100％（699／699）和99.6％（696／699），对43株酵母样菌的种鉴定符合率分别为90.7％（39／43）和79.1％（34／43）。这2种仪器均未鉴定出马尔尼菲青霉菌，但本研究利用MALDI-TOF MS技术建立了马尔尼菲青霉菌的蛋白质图谱。结论 Bruker Microflex MALDI-TOF MS对酵母菌的鉴定率高于Vitek 2 Compact全自动微生物分析系统，且操作快速简便，成本低廉，准确率高，可用于临床微生物实验室常见酵母菌的常规鉴定。（中华检验医学杂志，2015，38：382-386）

Objective To evaluate and compare yeast identification ability between Bruker Microflex matrix-assisted laser desorption ionization-time of flight mass spectrometry（ MALDI-TOF MS） and Vitek 2 Compact automatic microbial analysis system.Methods Retrospective study.Totally 742 strains of yeast isolated from clinical specimens during March 2013 to March 2014 in Xinhua Hospital, Shanghai Jiao Tong University School of Medicine were identified by Bruker Microflex MALDI-TOF MS and Vitek 2 Compact automatic microbial analysis system simultaneously.The strains with discordant results were validated by gene sequencing.Results The coincidence rate of 699 Candida identified by Bruker Microflex MALDI-TOF MS or Vitek 2 Compact system was 100.0%（699/699） and 99.6%（696/699） to the species level, respectively and the coincidence rate of 43 yeast-like fungi strains identified was 90.7%（39/43） and 79.1%（34/43） to the species level, respectively.Penicillium marneffei could not be identified by both two instruments, but protein profile of Penicillium marneffei by MALDI-TOF MS was established.Conclusions The coincidence rate of yeast identified by Bruker Microflex MALDI-TOF MS is higher than that of Vitek 2 Compact system.Using Bruker Microflex MALDI-TOF MS to identify yeast especially Candida and yeast-like fungus is fast, simple, low-cost, accurate, and it can be used in routine work of ordinary yeast identification in clinical microbiology laboratory.