皮质抑素在慢性阻塞性肺疾病大鼠中的变化及其意义

Role of cortistain alternation in rats with chronic obstructive pulmonary disease

目的 探讨皮质抑素在慢性阻塞性肺疾病（慢阻肺）大鼠中的变化及其意义.方法 16只健康雄性SD大鼠按随机数字表法随机分为对照组、慢阻肺组各8只;慢阻肺组给予被动烟雾吸入,对照组呼吸清洁空气.4个月后测定大鼠肺功能,支气管肺泡灌洗进行细胞学分类计数,取肺组织切片进行HE染色,观察肺组织病理形态改变.采用放免法检测大鼠血浆、支气管肺泡灌洗液（BALF）及肺组织中皮质抑素的表达水平.结果 慢阻肺组气道阻力、BALF嗜中性粒细胞百分比均显著高于对照组[（0.584±0.021）比（0.653 ±0.062） mmHg·ml-1·s-1、1.49％比0.41％]（均P＜0.05）;慢阻肺组呈现明显的气道炎症和肺气肿改变;慢阻肺组血浆、BALF、肺组织中皮质抑素均显著高于对照组[（526 ±76）比（453±41） pg/ml、（819±100）比（619±101） pg/ml、（41±10）比（20±7）pg/mg]（均P ＜0.05）.结论 皮质抑素在慢阻肺大鼠中表达增加,参与其气道炎症反应,可能具有抗炎保护作用.

Objective To explore the role of cortistatin （CST） in rats with chronic obstructive pulmonary disease （COPD）.Methods A total of 16 healthy male Sprague-Dawley rats were randomly divided into control and COPD model groups （n =8 each）.The COPD model group received an inhalation of passive smoking while the control group was treated normally.After 4 months,pulmonary function was measured.The differential cell counts of bronchoalveolar lavage fluid （BALF） were detected.Morphology and pathology of lungs were studied via hematoxylin and eosin staining.The levels of CST in lung tissue,serum and BALF were detected by radioimmunoassay.Results Compared with the control group,the COPD group had obviously higher airway resistance and neutrophil ratio in BALF [（0.584 ±0.021） vs （0.653 ± 0.062） mmHg · ml-1 · s-1,1.49% vs 0.41%,both P 〈 0.05].And there were significantly serious inflammation and emphysema in lung tissues.Moreover,the levels of CST increased in serum,BALF and lung tissues [（526 ±76） vs （453 ±41）pg/ml,（819 ± 100） vs （619 ± l01）pg/ml,（41 ± 10） vs （20 ±7）pg/mg,all P 〈 0.05] respectively as compared with the controls.Conclusion An elevated level of CST may compensate to exert anti-inflammation effects in COPD rats.