摘要
Background： Hydrogen sulfide （H,S） plays a protective role in chronic hemodialysis （CHD） patients. In this study, we further investigate the relationship between H,S and conventional protein kinase CβII （cPKCβII） in CHD patients with uremic accelerated atherosclerosis （UAAS）. Methods： A total of 30 healthy people, 30 CHD patients without AS and 30 CHD patients with AS （CHD ＋ AS） were studied. Plasma H,S was measured with a sulfide sensitive electrode, and cPKCβ11 membrane translocation was detected by Western blotting. Results： Plasma H2S in CHD ＋ AS group was significantly lower than that in CHD patients, cPKCβII membrane translocation in CHD ＋AS group increased significantly compared with CHD group. Plasma H,S concentration was negatively correlated with cPKCβ11 membrane translocation in CHD ＋ AS patients. Conclusions： These findings suggest a possible linkage between H,S metabolism and cPKCβ11 activation, which may contribute to the development of UAAS in CHD patients.
Background： Hydrogen sulfide （H,S） plays a protective role in chronic hemodialysis （CHD） patients. In this study, we further investigate the relationship between H,S and conventional protein kinase CβII （cPKCβII） in CHD patients with uremic accelerated atherosclerosis （UAAS）. Methods： A total of 30 healthy people, 30 CHD patients without AS and 30 CHD patients with AS （CHD ＋ AS） were studied. Plasma H,S was measured with a sulfide sensitive electrode, and cPKCβ11 membrane translocation was detected by Western blotting. Results： Plasma H2S in CHD ＋ AS group was significantly lower than that in CHD patients, cPKCβII membrane translocation in CHD ＋AS group increased significantly compared with CHD group. Plasma H,S concentration was negatively correlated with cPKCβ11 membrane translocation in CHD ＋ AS patients. Conclusions： These findings suggest a possible linkage between H,S metabolism and cPKCβ11 activation, which may contribute to the development of UAAS in CHD patients.
基金
This work was supported by grants from National Natural Science Foundation of China （No. 81200543）, Beijing Natural Science Foundation （No. 7142057）, Beijing Municipal Health Bureau High-level Medical Professionals Promotion Project （No. 2013-3-016） and Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support （No. ZYLX201408）.
