摘要
目的研究体外上调及下调miR-133a对滋养层细胞系HTR8-SVneo的粘附及侵袭功能的影响。方法将培养的HTR8-SVneo细胞根据脂质体转染因素分为对照组(转染空质粒)、上调组(转染mimics)、下调组(转染inhibitor),通过荧光定量PCR法检测各组miR-133a的表达水平,采用MTT法检测细胞粘附能力的变化,同时运用Transwell实验观察细胞侵袭能力的变化。结果与对照组比较,miR-133a上调组细胞黏附能力显著下降,miR-133a下调组细胞黏附力显著升高(P均<0.05);与对照组比较,miR-133a上调组细胞侵袭力显著降低,miR-133a下调组细胞侵袭力显著升高(P均<0.01)。结论 miR-133a在调控滋养层细胞粘附能力和侵袭能力方面起着重要作用。
Objective:To study the effect of up-regulation or down-regulation of miR-133 aon the adhesion and invasion function of trophoblast cell line HTR8-SVneo in vitro.Methods:According to the liposome transfection factors,the cultured HTR8-SVneo cells were divided into control group(transfect with empty plasmid),up-regulation group(transfect with mimics),downregulation group(transfect with inhibitor).The expression of miR-133 aafter transfection was detected by fluorescence microscopy and fluorescence quantitative PCR.The change of cell adhesion ability and invasion ability were detected by MTT method and Transwell experiment respectively.Results:The adhesion ability was significantly decreased in up-regulation group and increased in downregulation group compared with the control group(P〈0.05).The invasion ability was significantly decreased in up-regulation group and increased in down-regulation group compared with the control group(P〈0.01).Conclusions:miR-133 apays an important role on adhesion and invasion ability of trophoblast cell line.
出处
《生殖医学杂志》
CAS
2015年第6期477-480,共4页
Journal of Reproductive Medicine
基金
国家自然科学基金面上项目(81370710)
陕西省统筹创新项目(2013KTCL03-07)