miR-133a对滋养层细胞系HTR8-SVneo的粘附及侵袭功能的影响

Effect of miR-133a on adhesion and invasion function of trophoblast cell line

目的研究体外上调及下调miR-133a对滋养层细胞系HTR8-SVneo的粘附及侵袭功能的影响。方法将培养的HTR8-SVneo细胞根据脂质体转染因素分为对照组(转染空质粒)、上调组(转染mimics)、下调组(转染inhibitor),通过荧光定量PCR法检测各组miR-133a的表达水平,采用MTT法检测细胞粘附能力的变化,同时运用Transwell实验观察细胞侵袭能力的变化。结果与对照组比较,miR-133a上调组细胞黏附能力显著下降,miR-133a下调组细胞黏附力显著升高(P均<0.05);与对照组比较,miR-133a上调组细胞侵袭力显著降低,miR-133a下调组细胞侵袭力显著升高(P均<0.01)。结论 miR-133a在调控滋养层细胞粘附能力和侵袭能力方面起着重要作用。

Objective：To study the effect of up-regulation or down-regulation of miR-133 aon the adhesion and invasion function of trophoblast cell line HTR8-SVneo in vitro.Methods：According to the liposome transfection factors,the cultured HTR8-SVneo cells were divided into control group（transfect with empty plasmid）,up-regulation group（transfect with mimics）,downregulation group（transfect with inhibitor）.The expression of miR-133 aafter transfection was detected by fluorescence microscopy and fluorescence quantitative PCR.The change of cell adhesion ability and invasion ability were detected by MTT method and Transwell experiment respectively.Results：The adhesion ability was significantly decreased in up-regulation group and increased in downregulation group compared with the control group（P〈0.05）.The invasion ability was significantly decreased in up-regulation group and increased in down-regulation group compared with the control group（P〈0.01）.Conclusions：miR-133 apays an important role on adhesion and invasion ability of trophoblast cell line.