雄激素受体基因CAG多态性对前列腺癌恶性程度的影响

Effect of androgen receptor gene (CAG)_n polymorphism repeats on the severity of prostate cancer

目的检测正常人群、前列腺癌（PCa）低危和高危组雄激素受体（AR）基因（CAG）n微卫星多态性,探讨该重复序列长度对PCa恶性程度的影响。方法应用PCR和双链循环DNA测序技术,对98例正常人群（对照组）和95例经前列腺穿刺活检证实为PCa的患者,进行外周血标本（CAG）n串联重复序列检测,分析该微卫星多态性与PCa恶性程度的影响。结果 PCa组CAG重复序列为15-31,平均（23.21±3.07）;参照组重复序列为14-34,平均（24.20±3.50）,两者比较差异具有显著统计学意义（P〈0.05）。根据PCa患者肿瘤Gleason评分进行分级,≤7分为低危,共68例,≥8分为高危,27例。低危组的CAG重复序列为18-31,平均（23.65±2.92）,高危组CAG重复序列为15-27,平均（22.11±3.21）,两者比较差异具有统计学意义（P〈0.05）,高危组与参照组比较差异具有显著统计学意义（P〈0.01）。结论受试者AR基因（CAG）n重复序列呈现多态性,该重复序列长度与PCa的发生有关,较短的CAG重复序列预示发生PCa的可能性较大,恶性程度更高。

Objective To screen the （CAG）. polymorphism of androgen receptor （AR） gene in healthy men, patients with mild prostate cancer （PCa） and severe PCa, and to explore the relationship between the polymorphism with PCa severity. Methods A total of 98 healthy men （control group） and 95 PCa patients were enrolled. The length of AR gene CAG short tandem repeat （CAG-STR） sequences were detected by PCR and dsDNA cycle sequencing, and the effect of （CAG）.polymor- phism on PCa severity was analyzed. Results The number of CAG repeats for the PCa group and the control group were 15-31 （23.21±3.07） and 14 - 34 （24.20±3.50） respectively, with statistically significant difference between them （P 0.05）. According to PCa Gleason level, 68 cases were mild PCa （47） and 27 cases were severe PCa （98）. The number of CAG repeats for the mild and severe PCa groups were 18 - 31 （23.65±2.92） and 15 - 27 （22.11 ± 3.21 ）, respectively, with a significant difference. The number of CAG repeats in the severe PCa group was fewer than that in the control group （P〈0.01）. Conclusions The length of AR gene CAG repeat sequences present polymorphism in the subjects and may be one of the risk factors for PCa. Shorter CAG repeat sequences suggest severer PCa. KEY WORDS： genes; polymorphism; （CAG）n sequence repeat; androgen receptor; prostate cancer; severity degree