雪旺细胞及神经干细胞与Chitosan-PLGA导管的细胞相容性

Cytocompatibility research of schwann cells and neural stem cells co-seeded on electron spinning chitosan-PLGA

目的研究雪旺细胞(SCs)、神经干细胞(NSCs)与内置纵行排列纳米纤维丝的静电纺丝壳聚糖-聚羟基乙酸(Chitosan-PLGA)纳米导管材料的细胞相容性,探讨细胞在导管材料上的黏附及定向生长情况。方法实验分为SCs组、NSCs组、SCs-NSCs共培养组,3组细胞分别与Chitosan-PLGA纳米导管材料复合培养,四甲基偶氮唑蓝(MTT)比色法观察各组细胞存活率,扫描电镜下观察SCs、NSCs在Chitosan-PLGA上的生长。结果细胞存活率排序由高至低依次为共培养细胞组、SCs组、NSCs组。SCs及NSCs能贴附在Chitosan-PLGA内置纳米纤维丝上生长并沿纤维丝定向生长。结论雪旺细胞及神经干细胞共培养可促进细胞与Chitosan-PLGA材料的相容性。内置纵行排列的纳米纤维丝有引导细胞定向生长与迁移的作用。

Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells （SCs）and neural stem cells（NSCs）as well as cells adhesion and migration on the arranged nanofibers inside the lumen.Methods Experiment was divided into three groups：the SCs group,the NSCs group and the SCs-NSCs co-culture group.Cells in these groups were co-seeded with Chitosan-PLGA.The biocompatibility and absorba-bility of SCs and NSCs were detected by MTT assays.Scanning electron microscopy（SEM）was used to detect cells ad-hesion and migration on the axially arranged nanofibers inside the lumen.Results Cell survival rate of the co-culture group was the highest,the higher in the SCs group,and the lowest in the NSCs group.SCs and NSCs had good compati-bility and adhesion with Chitosan-PLGA,and the axial nanofibers in the lumen guided the growth of neurites in a particu-lar direction.Conclusion Co-culture of SCs and NSCs can facilitate cells compatibility and adhesion with 3D Chitosan-PLGA,and the axial arranged nanofibers within the lumen has the effect of directional guide cell growth and migration.