microRNA-130a在非小细胞肺癌中的表达和功能

The expression and function of microRNA- 130a in non- small cell lung cancer

目的:探讨microRNA-130a(miR-130a)在非小细胞肺癌中的表达情况,并对其功能和分子机制进行研究。方法:收集50例非小细胞肺癌肿瘤组织和相对应的正常肺组织,采用RT-PCR方法检测miR-130a的相对表达量,并分析其与临床病理资料的相关性。采用体外转染法将miR-130a抑制剂瞬时转染到A549细胞后,应用real-time PCR检测A549细胞中miR-130a的表达情况。CCK8实验检测细胞转染后的增殖变化。Western blot检测转染后细胞中Smad4的表达变化。结果:肺癌组织中miR-130a的表达水平明显高于正常肺组织(P<0.05);miR-130a的表达水平与肿瘤大小、淋巴结转移和临床分期明显相关(P<0.05)。与对照组比较,A549细胞转染miR-130a抑制剂后miR-130a表达水平明显下调,细胞增殖率明显下降,差异具有统计学意义(P<0.05);转染miR-130a抑制剂后,肺癌细胞中Smad4的表达水平明显上调。结论:miR-130a在NSCLC组织中表达上调,可能部分通过下调Smad4的表达来促进肺癌细胞的增殖和转移。

Objective：To investigate the exPression of miR-130a in Patients with non-small cell lung cancer （NSCLC）and to analyze the function and mechanism of miR-130a. Methods：The exPression levels of miR-130a in 50 tumor tissues and Paired normal tissues of NSCLC were detected by using RT-PCR. The relationshiP between the level of miR-130a and the Pathological characteristics was analyzed. miR-130a in vitro was transiently trans-ferred into A549 cells. The exPression level of miR-130a in A549 cells was detected by RT-PCR. Cell viability was analyzed by CCK8 assay. ExPression of Smad4 Protein was detected by Western blot. Results：The exPression level of miR-130a was uP-regulated in tumor tissues as comPared with normal lung tissues（P〈0. 05）. The exPression of miR-130a was associated with tumor size,lymPhatic metastasis,and TNM stages（P〈0. 05）. miR-130a in anti-miR-130a-transfected A549 cells was significantly decreased comPared with the control grouP（P〈0. 05）. The Proliferation of anti-miR-130a-transfected A549 cells was inhibited significantly. The exPression level of Smad4 was markedly uP-regulated after transfection（P〈0. 05）. Conclusion：The exPression of miR-130a was uP-regu-lated in NSCLC,miR-130a might Promote Proliferation and metastasis of lung cancer in Part by down-regulating the exPression of Smad4.