MiR-451在甲状腺乳头状癌中的表达及其临床诊断价值

The expression of miR-451 in thyroid papillary carcinoma and its value of the clinical diagnosis

目的:探讨miR-451在甲状腺乳头状癌中表达及其与临床诊断的关系。方法:收集2005年7月-2007年7月在上海交通大学医学院附属新华医院甲状腺外科收治的60例甲状腺乳头状癌患者的临床资料及对应的石蜡组织。用荧光定量PCR检测miR-451,比较其在甲状腺癌组织和癌旁组织中的表达差异。结果:MiR-451的表达水平甲状腺癌组织显著低于癌旁组织[(38.79±27.26)vs(12.62±13.44),P=0.000]。此外,miR-451表达水平与患者的性别、年龄、肿瘤大小、肿瘤位置均无显著相关性。但伴有淋巴结转移的甲状腺乳头状癌组织中miR-451表达水平显著低于不伴有淋巴结转移的甲状腺乳头状癌组织(P=0.006)。结论:MiR-451能区分甲状腺乳头状癌癌旁组织与癌组织,以及判断甲状腺乳头状癌患者是否伴有淋巴结转移,可能作为辅助的临床诊断指标。

Objective：To study the expression of miRNA-451 and its relationship with the clinical diagnosis in thyroid papillary carcinoma.Methods：From July 2005 to July 2007,we collected clinical data and pathological tissue of 60 patients with thyroid papillary carcinoma at Xinhua Hospital Affiliated the Shanghai Jiaotong University School of Medicine.Detecting miR-451 by using fluorescence quantitative PCR,comparing the differential expression between thyroid carcinoma and normal tissue.Results：Compared the adjacent tissues of cancer,the expression level of miR-451 in thyroid cancer tissues was significantly lower than that of normal tissues [（38.79 ±27.26） vs （12.62 ± 13.44）,P =0.000].In addition,the expression level of miR-451 had no significant correlation with patients＇ age,gender,tumor size,but was significantly lower in which with lymph node metastasis than in which without lymph node metastasis （P =0.006）.Conclusion：MiR-451 can distinguish cancer tissues from adjacent normal tissues,may be used as the auxiliary clinical diagnostic criteria with or without lymph node metastasis in papillary thyroid cancer.