PTPRO基因启动子甲基化与卵巢癌淋巴结转移的相关性

Clinical relationship of PTPRO gene promoter methylation with lymphnode metastasis in ovarian carcinoma

目的:分析卵巢癌中受体O型蛋白酪氨酸磷酸酶(PTPRO)基因启动子甲基化状态及临床意义。方法:应用甲基化特异性PCR检测PTPRO基因在50例卵巢癌组织和27例正常卵巢组织中的甲基化状态并结合临床病理资料进行分析。结果:在卵巢癌组织中PTPRO甲基化阳性率为66%(33/50),正常卵巢组织未出现PTPRO甲基化;PTPRO甲基化与肿瘤淋巴结转移与FIGO分期密切相关(P<0.05)。结论:PTPRO基因甲基化可能是参与卵巢癌发展的重要分子事件。

Objective：To analyze the methylation status of the PTPRO gene promoter in human ovarian carcinoma.Methods：Methylation-specific PCR was performed to observe the PTPRO promoter methyation in 50 cases of ovarian carcinoma tissues and 27 normal ovarian tissues.The relationship between PTPRO methyation and clinical pathological parameters was analyzed.Results：Methylated PTPRO was observed in 66％ of primary tumors （33/50）,but not in any normal tissue.PTPRO methylation was significantly correlated with lymphnode metastasis and FIGO staging of tumors （P ＜ 0.05）.Conclusion：PTPRO methylation may be an essential molecular event involved in development of ovarian carcinoma.