热休克蛋白70的表达与HBV相关肝细胞癌临床病理学特征的相关性分析

Analysis of association between HSP70 expression in tumor tissues and clinicopathological characteristics of HBV-related hepatocellular carcinoma

目的：分析 HBV 相关肝细胞癌（HCC）肿瘤组织中热休克蛋白（HSP）70表达水平与 HCC 临床病理学特征的相关性。方法通过基因表达数据库（GEO）获取资料，对 HSP70基因表达与 HCC 临床病理学特征进行统计学分析。分类变量采用频数（构成比）进行统计描述，统计分析采用χ2检验或 Fisher 确切概率法。HSP 与 HCC 临床病理学特征相关性分析采用单因素及多因素 Logistic 回归分析，单因素分析显示 P ＜0．10的变量纳入多因素分析。结果HSPA4L、HSPA6、HSPA13高表达患者 HCC 主瘤体＞5 cm 的比率明显高于低表达组（P 值均＜0．05）；HSPA1A、HSPA6低表达组合并肝硬化的比率明显低于高表达组（P 值均＜0．05）；HSPA5、HSPA13高表达组 AFP ＞300 ng／ml 的比率明显高于低表达组（P 值均＜0．01）。合并肝硬化和（或）主瘤体＞5 cm 的 HCC 患者生存率明显降低（P 值均＜0．05）。经 Logistic 多因素分析，HSPA4L 高表达可促进 HCC 瘤体生长[OR ＝1．019，95％可信区间（95％CI）：1．006～10．032，P ＝0．003]；HSPA1B 高表达的 HCC 患者合并多发结节的风险较大（OR ＝1．002，95％CI：1．000～1．004，P ＝0．003）。结论HSPA4L 及 HSPA1B 的表达与 HCC 临床病理学特征存在一定的相关性，可能为 HCC 治疗靶点及预后预测因子。

Objective To investigate the relationship between heat shock protein 70 （HSP70）expression and clinicopathological character-istics of hepatitis virus B （HBV）-induced hepatocellular carcinoma （HCC）.Methods Statistical analysis was conducted to explore the association between the data of HSP70 obtained from the gene expression omnibus （GEO）and the clinicopathological characteristics of HCC.As for categorical data,frequency （proportion）was used to describe and chi-square test or Fisher′s exact test was applied to ana-lyze.Univariate and multivariate logistic regression analyses were used to investigate the relationship between HSPexpression and the clini-copathological characteristics of HCC.The variables with a Pvalue less than 0.10 were included for subsequent multivariate analysis.Re-sults 1）The percentage of the HCC patients whose primary tumor sizes were more than 5 cm was significantly higher in the group with high expression of HSPA4L,HSPA6,and HSPA13 than in the group with low expression （P〈0.05）;in comparison with the group with high ex-pression of HSPA1A and HSPA6,the group with low expression had a significantly lower percentage of patients accompanied by liver cirrho-sis （P〈0.05）;there was a significantly higher percentage of patients with alpha-fetoprotein （AFP）levels more than 300 ng/ml in the group with high expression of HSPA5 and HSA13 compared with the group with low expression （P〈0.01）.2）The survival rates of HCC patients with liver cirrhosis and /or primary tumor that was more than 5 cm significantly decreased （P〈0.05）.3）Highly expressed HS-PA4L might promote the growth of HCC （OR =1.019,95% CI：1.006-10.032,P=0.003）;the patients with high expression of HSPA1B might have a higher risk of developing multiple nodules （OR =1.002,95%CI：1.000-1.004,P=0.003）.Conclusion The expression of HSPA4L and HSPA1B is,to some extent,associated with the clinicopathological characteristics of HCC,which might be a therapeutic tar-get and prognostic predictor for HCC.