氢醌对K562细胞着色性干皮病基因D转录及表达的影响

Effects of Hydroquinone on Transcription and Expression of XPD Gene in K562 Cells

目的:探讨苯代谢物氢醌(HQ)对人白血病细胞株K562细胞中着色性干皮病基因D(XPD)mRNA转录及蛋白表达的影响。方法:分别用终浓度为0、15、30、60μmol/L HQ溶液处理K562细胞48 h后,单细胞凝胶电泳实验检测细胞DNA损伤效应,Taqman探针实时荧光定量PCR法检测XPD基因mRNA转录水平,蛋白印迹法分析XPD基因蛋白表达水平,观察荧光显微镜下细胞的尾矩,计算mRNA及蛋白的相对表达量。结果:不同浓度HQ处理后,各组细胞尾矩与0μmol/L HQ组比较,差异有统计学意义(P<0.05);不同浓度HQ处理后,K562细胞中XPD基因mRNA和蛋白相对表达量与0μmol/L HQ组比较,差异无统计学意义(P>0.05)。结论:HQ处理K562细胞48 h后,细胞中DNA有明显的损伤效应,但XPD基因mRNA及蛋白无变化。

Objective： To explore effects of different concentration of hydroquinone（ HQ） on mRNA transcription and protein expression of xeroderma pigmentosum group D（ XPD） in Human leukemia cell line K562 cells. Methods： K562 cells were treated and contaminated with 0,15,30,60 μmol /L hydroquinone for 48 h,respectively. The DNA damage effect of the cells exposed to hydroquinone was detected by single cell gel electrophoresis. The mRNA transcription of XPD was detected by Taqman probe real-time fluorescence quantitative PCR. The protein expression of XPD was detected by Western blot. Results： In DNA damage effect experiment,there existed significant differences in the tail moment between contamination groups and control group（ 0 μmol / L hydroquinone）（ P 0. 05）.In experiments of mRNA transcription of XPD and protein expression of XPD,there was no significant difference in mRNA transcription of XPD and protein expression of XPD between contamination groups and control group（ P 0. 05）. Conclusion： After K562 cells are treated with hydroquinone for 48 h,the DNA damage effect of K562 cells is obvious. But the change of mRNA transcription and protein expression of XPD can not be found.