碱性成纤维生长因子对脂肪干细胞分化为心肌细胞的影响

Influence of Basic Fibroblast Growth Factor on Differentiation of Adipose Derived Stem Cells into Cardiomyocytes

【目的】探讨碱性成纤维生长因子(b FGF)对脂肪干细胞(ADSC)分化为心肌细胞的影响。【方法】从SD大鼠中提取、分离、培养、鉴定ADSC后,评价ADSC分化能力。将成功建立心肌梗死(MI)模型的60只大鼠随机分4组(n=15/组):PBS组、b FGF组、ADSC组及b FGF+ADSC组。建立心梗1周后分别注射PBS、b FGF、ADSC及b FGF+ADSC,4周后用心脏超声测量心梗大鼠的左室射血分数(EF),取大鼠心脏检测左室梗死面积、微血管密度(MVD)及ADSC分化。【结果】成功分离ADSC,流式细胞分析显示大多数ADSC表达CD29和CD90,不表达CD34和CD45。ADSC能被诱导分化成成脂、成骨细胞。b FGF+ADSC组及ADSC组的EF高于PBS组和b FGF组(P<0.01),b FGF+ADSC组的EF高于ADSC组(P<0.01),PBS组和b FGF组的EF差别无统计学意义(P=0.33)。b FGF+ADSC组及ADSC组的梗死面积小于PBS组和b FGF组(P<0.01),b FGF+ADSC组的梗死面积小于ADSC组(P<0.01),PBS组和b FGF组的梗死面积差别无统计学意义(P=0.28)。四组中b FGF+ADSC组的MVD最大(P<0.01)。ADSC可分化为c Tn T阳性细胞、SMA阳性细胞及fⅧ阳性细胞。【结论】b FGF可促进ADSC分化成心肌细胞和新生血管,改善心脏功能。

[Objective] To study the influence of basic fibroblast growth factor （bFGF） on differentiation of adipose derived stem cells （ADSC） into cardiomyocytes.[Methods] ADSC isolated from SD rats were cultured and characterized,then the differentiation of ADSC was verified.60 rats with myocardial infarction （MI） were randomized into 4 groups （group PBS,group bFGF,group ADSC,and group bFGF＋ADSC,（n =15/group）.PBS,bFGF,ADSC,and bFGF＋ADSC were injected along peri-infarct zone at four injected foci respectively.Ejection fraction （EF）,infarcted area,microvessel density （MVD） and ADSC differentiation were evaluated.[Results] ADSC were successfully isolated and most ADSC showed positive CD90,CD29 and negative CD45,CD34 by fluorescence-activated cell sorting （FACS）.ADSC could be differentiated into adipogenic and osteogenic cells.Injections of ADSC or bFGF＋ADSC significantly increased EF compared with PBS or bFGF,with implantation of bFGF＋ADSC highest （P ＜ 0.01）.The infarcted area was significantly reduced by the injection of bFGF＋ADSC compared with the injections of PBS,or bFGF,or ADSCs （P ＜ 0.01）.There was no difference in infarcted area between group PBS and group bFGF （P =0.28）.Injections of bFGF＋ADSC significantly increased MVD compared with PBS,bFGF,or ADSC,with implantation of bFGF＋ADSC highest （P ＜ 0.01）.ADSC could differentiate into cTnT positive cells orSMA positive and fⅧ positive cells.[Conclusion] bFGF was able to improve heart function by promoting the differentiation of ADSC into cardiomyocytes and angiogenesis.