不同浓度 hTNFα对软骨细胞及其基质相关基因表达的影响

Effects of different concentrations of hTNFα on chondrocytes and matrix-associated gene expression

目的探讨软骨细胞在肿瘤坏死因子(TNFα)作用下对其分泌基质蛋白及相关基因mRNA表达的影响。方法体外培养的小鼠软骨细胞用25,50,100 ng/ml的h TNFα干预,不加入TNFα作为对照,通过RT-q PCR检测h TNFα对软骨细胞分泌基质蛋白及相关基因mRNA表达的影响。结果 h TNFα能降低软骨细胞分泌的Ⅱ型胶原及刺激促Ⅱ型胶原分泌的SOX9基因的表达;对基质可提高基质酶MMP-9,MMP-13,ADAMTS-4和ADAMTS-5基因的表达,提高炎性因子IL-1α,TGFβ,并增加凋亡基因BAX,caspase3的表达。结论 h TNFα通过不同途径影响软骨细胞及基质相关基因的表达,从而促进了关节软骨的降解。

Objective To investigate the effect of tumor necrosis factor （ TNFα） on the secretion of matrix protein and mRNA expression of related genes in cartilage cells.Methods Mouse chondrocytes were cultured in vitro and were additioned with 25, 50, and 100 ng/ml of hTNFα.No hTNFαcultures were as control.mRNA expression of related genes in chondrocytes was detected using RT-qPCR.Results hTNFαreduced type II collagen secretion in cartilage cells and stimulated SOX9 gene expression that stimulates pro-II collagen secretion.It increased gene expression of MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 in the matrix.It also increased inflammation factors IL-1αand TGFβ, and increased apoptotic gene expression of BAX and caspase 3. Conclusion hTNFαaffects the expression of matrix-related genes in cartilage cells by different ways, thus stimulating the degradation of articular cartilage.