两品系小鼠局部淋巴结试验结果比较

The comparative study of two strains on results of Local lymph node assays

目的分析比较BALB/c和CBA小鼠局部淋巴结试验结果。方法将已知致敏性的4种化学物(2,4-二硝基氯苯、丁子香酚、己基肉桂醛和异丙醇)和未知致敏性的3种化妆品对BALB/c和CBA小鼠连续染毒3d,第5天腹腔注射示踪剂BrdU,第6天分离颌下淋巴结制取单细胞悬液,用ELISA试盒检测细胞增殖。结果2,4-二硝基氯苯、丁子香酚、己基肉桂醛和3号烫发剂两品系结果均呈致敏阳性,3种化学物EC1.6值分别为0.08%(极强)、4.02%(中)、6.68%(中)(BALB/c)和0.07%(极强)、6.08%(中)、8.89%(中)(CBA);异丙醇、1和2号化妆品两品系结果均为致敏阴性,SI均小于1.6。结论两品系小鼠局部淋巴结试验结果较一致,BALB/c可替代CBA小鼠应用于化学物和化妆品的致敏性评价。

Objective To analyze and compare results of local lymph node assays（LLNA） between BALB/c and CBA mice.Methods 4 chemicals（2,4-dinitrochlorobenzene,eugenol,hexyl cinnamic aldehyde and isopropanol） and 3cosmetics were applied to the dorsum of both ears of Balb/c and CBA mice for three consecutive days.Brd U solution was injected inter-peritoneally on day 5.On day 6,the bilateral draining auricular lymph nodes were excised and made into single cell suspension,the lymph cell proliferation was measured by Brd U ELISA kit.Results 2,4-dinitrochlorobenzene,eugenol,hexyl cinnamic aldehyde and NO.3 perm agent pretended positive for both strains,EC1.6 values of three chemicals were found to be 0.08%（very strong）,4.02%（moderate）,6.68%（moderate） and 0.07%（very strong）,6.08%（moderate）,8.89%（moderate） for BALB/c and CBA mice respectively.Isopropanol,NO.1 and NO.2cosmetics pretended to be non-sensitizers with SI 1.6 for both strains.Conclusion This study showed that BALB/c mouse was essentially equal to CBA for LLNA： Brd U-ELISA,which suggested that BALB/c mouse was a good alternative for CBA used in chemicals and cosmetics allergenic evaluation.