新疆维吾尔族人群过氧化物酶体增殖物激活受体基因相关SNP位点的多态性

SNP Polymorphism of PPARG Gene in Xinjiang Uygur Population

对过氧化物酶体增殖物激活受体基因(PPARG)的31个SNP位点进行群体遗传学分析,利用质谱检测技术检测PPARG基因的31个SNPs位点多态性,并根据质谱峰图判读样本目标位点基因型,统计分析31个SNP位点的基因型和等位基因的分布频率,利用x2检验确定筛选的SNP位点是否符合Hardy-Weinberg平衡定律。结果发现31个SNP位点中,23个位点的次等位基因分布频率MAF≥0.05,在新疆维吾尔族人群中具有多态性,其他8个SNP位点的MAF<0.05,没显示多态性。基因型和等位基因频率在男女两组间均无统计学差异(P>0.05),表明这些位点等位基因分布不存在性别差异。

To get the data of allele and genotype frequencies of 31 SNP loci in PPARG gene, the mass spectrometric use for detection of 31 SNPs loci of PPARG gene and interpretation genotype of 31 SNPs with the mass spectrum peak figure.The x^2（Chi-square test）is used to determine filtered SNPs whether or not accord with Hardy-Weinberg equilibrium. Among the 31 SNP loci,23 SNP loci were MAF≥0.05,showing polymorphism in Xinjiang Uygur population.while the others were below MAF 〈0.05. There were not any gender difference at genotype frequency characteristics.