基于核酸适体和胶体金技术构建凝血酶的DNA逻辑门

DNA Logic Gate for Thrombin Based on Aptamer and Gold Nanoparticle Technology

DNA逻辑门在分子计算机和分子水平上的计算技术中具有重要作用。我们通过设计核酸适体与凝血酶特异结合导致胶体金团聚的方法,构建了凝血酶的DNA逻辑门。凝血酶作为输入信号与其核酸适体结合,将监测探针上的胶体金结合位点释放,两个核酸功能化修饰的胶体金同时结合到监测探针上,引起胶体金团聚,实现信号输出。根据这个设计原理,成功构建了蛋白质凝血酶的YES逻辑门。

DNA logic gate plays an important role in the molecular computer and a molecular level of computer technology. Based on the binding of the aptamer and thrombin and assemble of functionalized gold nanoparticles, a DNA logic gate was constructed. When added thrombin as the input, the binding of thrombin and its aptamer results to release the site of the gold nanoparticle site in a detection probe, in which two gold nanoparticles hybridizes with the probe and causes the assemble of gold nanoparticles and realizes the output signal of logic gate. According to the design principle, the YES logic gate for protein thrombin was successfully constructed.