胡黄连酚苷提取工艺研究

Extraction process of androsin

应用医药用高浓度乙醇回流提取药材胡黄连的方法,以胡黄连酚苷得量和提取物中胡黄连酚苷含量为主要指标,采用L9(34)正交设计优选胡黄连酚苷最佳提取工艺条件。由正交试验结果得出最佳提取方案为A1B3C3,即用药材量20倍的95%乙醇,分3次回流提取,共4.5 h,其中第一次加10倍量回流2 h,第二次6倍量回流1.5 h,第三次4倍量回流1 h。

We employed medical high-concentration alcohol reflux to extract androsin. We optimized extraction conditions with L_9 （3^4） orthogonal design and androsin yield and content as main indexes. Experimental results show that the optimal extraction scheme is A, B3 C3,3 times reflux extraction for 4.5 h with 95% alcohol of 20 times medical amount （10 times amount reflux for 2 h for the first time, 6 times amount reflux for 1. 5 h for the second time, and 4 times amount reflux for 1 h for the third time）.