葡萄霜霉病拮抗细菌BMJBN02的筛选及其抑菌效果研究

Screening and identification of antagonistic bacteria strain BMJBN02 and its effect on grape downy mildew

采用平板观察法,获得到6株对葡萄霜霉病具有抑制作用的菌株,对其代谢产物进行检测,发现菌株BMJBN02在纤维素平板、几丁质平板和β-1,3-葡聚糖平板上产生的水解圈半径最大。通过对菌株BMJBN02的形态、生长特性、生理生化反应、16S r DNA序列测定和系统发育分析,鉴定该菌株为巨大芽孢杆菌(Bacillus.megaterium spp.)。叶盘法检测发现,经菌株BMJBN02粗提液处理的叶片,霉层的面积明显减少,叶片发病率从100%降低到42.3%,病情指数从65.3降为18.6,抑菌效果显著。

6 strains exhibiting antagonistic activities against grape downy mildew blight were obtained by the method of plate observation. By the detection＇ of metabolites, the strain BMJBN02 was provided with the optimum efficiency of cellulase,chitinase and β-1,3-glucanase, and its hydrolysis circle on the plant was the largest. The results showed the strain BMJBN02 was identified as Bacillus. megaterium,through analyzing its morphological,physiological and biochemical characteristics, 16S rDNA sequence and phylogenetics. Using the leaf disc for screening, it was significant that area of the mold layer significantly reduced inhibitory effect in leaves treated by strain BMJBN02, with the disease incidence reduced from 100% to 42.3% and disease stage reduced from 65.3 to 18.6.