Toll样受体9和CD_(123)在寻常型银屑病患者皮损中的表达

Expression of Toll-like Receptors 9 and CD_(123) Plasmacytoid Dendritic Cells in Lesions of Patients with Psoriasis Vulgaris

目的探讨Toll样受体9(TLR9)和CD123在寻常型银屑病患者皮损中的表达及意义。方法选择2012年6月至2013年4月兖州市人民医院收治的26例寻常型银屑病患者为试验组,另选23例健康体检者为对照组,分别按照常规皮肤手术采集躯干部皮损样本进行活检,并采用免疫组织化学S-P法及反转录-聚合酶链反应(RT-PCR)检测其组织中的CD123及TLR9表达情况,分析其过表达与寻常型银屑病患者发病的机制。结果试验组免疫组织化学S-P法CD123及TLR9阳性表达率均为100%,其表达水平分别为(10.1±2.3)个/mm3和(14.4±2.0)个/mm3,而对照组表达水平分别为(1.5±0.6)个/mm3,(2.2±1.0)个/mm3,但无阳性表达;试验组RT-PCR法TLR mRNA表达水平高于对照组[(1.32±0.08)比(0.87±0.04)],差异有统计学意义(P<0.01)。结论浆细胞样树突状细胞可能参与激发自身免疫T细胞活化和增殖,是寻常型银屑病发病的一个重要机制之一。

Objective To investigate the expression of toll-like receptors 9（ TLR9） and CD123 plasmacytoid dendritic cells in skin lesions of patients with psoriasis vulgaris and its significance. Methods A total of 26 patients with psoriasis vulgaris admitted in People＇ s hospital of Yanzhou City from Jun. 2012 to Apr.2013 were selected as the experimental group,another 23 healthy people underwent physical examination were selected as the control group. Skin lesion samples from the bodies of all the cases were collected by the method of general skin surgeries and received biopsies,and the expression of CD123 and TLR9 in the samples were detected by S-P immunohistochemical method and reverse transcription-polymerase chain reaction（ RT-PCR）,and the relationship between the overexpression of the two factors and the pathogenesis of psoriasis vulgaris was analyzed. Results The positive expression rate of CD123 and TLR9 in the experiment group detected by S-P immunohistochemical method were both 100%,the expression mean values of CD123 and TLR9 were respectively（ 10. 1 ± 2. 3） figures / mm3,（ 14. 4 ± 2. 0） figures/mm3,while in control group,the expression mean values of CD123 and TLR9 were respectively（ 1. 5 ± 0. 6） figures / mm3,（ 2. 2 ± 1. 0） figures / mm3,both without positive expression; the expression levels of TLR mRNA in experiment group and control group detected by RT-PCR method were（ 1. 32 ± 0. 08） figures / mm3and（ 0. 87 ± 0. 04） figures / mm3,respectively,the difference was statistically significant（ P〈0. 01）. Conclusion Plasmacytoid dendritic cells may be involved in stimulating the activation and proliferation of autoimmune T cell,which is an important mechanism of the incidence of psoriasis vulgaris.