狗牙根耐旱基因的表达谱分析

Expression Spectrum Analysis of Bermudagrass(Cynodon dactylon L.)Drought-Tolerant Gene

为了提高草坪草对干旱环境的适应能力,了解植物对干旱的响应特征,研究从已建立的草坪草狗牙根‘Tifway’(耐旱)和‘C299’(干旱敏感)干旱5和10 d应答SSH(suppression subtractive hybridization)4个文库中挑选出38个耐旱、干旱响应基因。采用半定量RT-PCR方法检测干旱胁迫下这些基因在‘Tifway’和‘C299’中的表达变化,比较它们在两品种间的表达差异。发现叶表面蜡质合成基因(CER1蛋白基因、固醇脱氢酶基因)、与耐旱相关的基因(脱水素基因)、与抗氧化相关基因(超氧化物歧化酶、脱氢抗坏血酸还原酶和2-半胱氨酸过氧化物酶基因)、和转录调控因子(EREBP-4like蛋白和WRKY)在‘Tifway’中的表达量显著高于‘C299’。这些结果说明了‘Tifway’比‘C299’更加耐旱的分子机制。

In order to improve the adaptation of turfgrass to drought stress and know the response of plant to drought,we examined the alternations of 38drought-tolerant or drought-regulated gene expressions under drought conditions.These 38 genes were selected from four libraries of suppression subtractive hybridization（SSH）which were established by ‘Tifway＇（drought-tolerant）and ‘C299＇（droughtsensitive）of bermudagrass responding drought tolerance for 5and 10 d.We compared the differences of gene expressions between‘Tifway＇and‘C299＇using semi-quantitative RT-PCR.The results showed that certain gene expressions,such as leaf surface wax synthesis genes（CER1 protein gene and sterol dehydrogenase gene）,drought tolerant gene（dehydration gene）,and oxidation resistance related gene（dismutase,dehydrogenation of ascorbic acid reductase and 2-cysteine peroxidase gene）,and transcription factor（EREBP-4like protein and WRKY）in ‘Tifway＇are significantly higher than that in ‘C299＇.It indicates that‘Tifway＇is more drought-tolerant than‘C299＇at molecular level.