FN和MMP-14蛋白在鼻咽癌中的表达及其临床意义

Expression and clinical significance of FN and MMP-14 protein in nasopharyngeal carcinoma

目的探讨鼻咽癌组织中纤维连接蛋白(FN)和基质金属蛋白酶14(MMP-14)的表达及其与临床病理特征的关系。方法选取56例鼻咽癌标本作为观察组,18例鼻咽炎标本作为对照组,采用SP免疫组织化学法检测两组标本中FN和MMP-14的阳性表达情况,并分析二者的关系及其临床意义。结果基底膜FN在鼻咽癌组织中的表达低于鼻咽炎组织,间质FN在鼻咽癌组织中的表达高于鼻咽炎组织(P<0.05或0.01),而MMP-14在鼻咽癌组织中的表达明显高于鼻咽炎组织(P<0.01);基底膜FN和MMP-14在鼻咽癌组织中的表达与年龄、性别无显著性相关(P>0.05),与淋巴结转移及临床分期相关(P<0.01);基底膜FN和MMP-14蛋白在鼻咽癌组织中的表达呈负相关(r=-0.478,P=0.000)。结论 MMP-14的高表达及FN的异常表达在鼻咽癌淋巴结转移及临床分期中可能具有重要作用。

Objective To investigate the expressions of fibronectin（ FN） and matrix metalloproteinases-14（ MMP-14） in nasopharyngeal carcinoma and their relationship with clinicopathologic characteristics. Methods 56 cases of nasopharyngeal carcinoma specimens were selected as observation group,and 18 cases of nasopharyngitis specimens as control group. SP immunohistochemical method was used to detect positive expressions of FN and MMP-14 of specimens in both groups. Meanwhile,the relationship between the expressions and clinical significance was also analyzed. Results In nasopharyngeal carcinoma tissues,the positive expression of basement membrance FN was significantly lower than that in the nasopharyngitis tissues,while that of interstitial FN was higher than that in nasopharyngitis tissues（ P〈0. 05 or 0. 01）,and the positive expression of MMP-14 was significantly higher than that in nasopharyngitis tissues（ P〈0. 01）. The positive expressions of basement membrance FN and MMP-14 had no significant correlation with gender and age（ P〉0. 05）,but they had close relationship with lymphatic metastasis and clinical stage（ P〈0. 01）. Besides,the expressions of basement membrance FN was negatively correlated with MMP-14 in nasopharyngeal carcinoma tissues（ r =-0. 478,P = 0. 000）. Conclusion High expression of MMP-14 and aberrant expression of FN may play an important role in lymph node metastasis and clinical staging of nasopharyngeal carcinoma.