米非司酮对创伤后应激障碍预防作用的实验研究

The preventive effects of mifepristone for posttraumatic stress disorder

目的建立较理想的创伤后应激障碍(PTSD)动物实验模型并评价米非司酮对PTSD的预防效果。方法建立大鼠捕食应激模型,随机分为对照组、模型组和干预组(高、中、低剂量亚组)每组8只。随后采用行为学实验,空间学习记忆能力实验评价米非司酮对PTSD发生的预防效果,同时观察米非司酮干预后大鼠皮质酮水平变化与脑组织细胞凋亡情况。结果米非司酮可改善大鼠应激后的活动习性改变、警觉水平过高、惊恐行为、环境适应能力下降、躲藏逃避反应等多种行为异常以及空间学习和记忆能力障碍,同时,米非司酮可升高大鼠应激后血清皮质酮水平,抑制大鼠脑组织细胞bcl-2表达。结论米非司酮可预防捕食应激大鼠PTSD症状发生。

Objective To explore mifepristone the preventive effect on PTSD（posttraumatic stress disorder）rats.Methods Rats of PTSD animal model were randomly divided into control group,model group and mifepristone（L/M/H）group which adminstered mifepristone.The behavior changes of rat were observed with open-field test,reject grip test and Morris water maze test and corticosterone level in rat blood serum was determined with ELISA.Immunohistochemistry was used to detect the expressions of bcl-2in the neurons.Results Mifepristone prevented the behavior changes after the rats were explored to the predator,such as behavioral habits,anxiety,defense,spatial learning and memory.RU38468 also increased cortisol level and decreased the expression of bcl-2.Conclusion This study provides a new idea that mifepristone may have the preventive effects for posttraumatic stress disorder.