摘要
目的:建立高效液相色谱法测定白背叶药材中大波斯菊苷含量的方法。方法:采用Gemini—C18色谱柱(4.6mm×250mm,5μm),以甲醇-0.1%磷酸水溶液(40:60)为流动相,柱温:25℃,流速:1.0mL·min,检测波长:336nm。结果:大波斯菊苷达到基线分离,在0.04486~0.8972/zg范围内线性关系良好(r=0.9993),大波斯菊苷平均加样回收率(n=6)为97.67%(RSD=1.94%)。结论:所建立的含量测定方法操作简单、快速、重复性好,可用于白背叶药材的质量控制。
Objective:To establish an HPLC method for determination of Apigenin-7-O-glucoside in Mallotus apelta. Methods: HPLC was performed on a Gemini-C18 analytical column (4.6minx 250 ram, 5μm) at 25 ℃ with methanol and 0.1% Phosphoric acid (40..60) as the mobile phase. Results: The baseline separation of Apigenin-7-O-glucoside was achieved The linear ranges of 0.04486-0.8972μg (r=0.9990). The average recovery(n=6) was 97.67% (RSD=1. 94 % ). Conclusion : The method of determination is proved to be simple, quick, repeatable, and can be used for quality control of Mallotus apelta.
出处
《亚太传统医药》
2015年第13期16-18,共3页
Asia-Pacific Traditional Medicine
基金
广西科技创新能力与条件建设项目(桂科攻10124008-15)
广西自然科学基金项目(2010GXNSFA013233)