瓜蒌皮药材质量标准研究

Quality Standard Study on Trichosanthis Pericarpium

目的：完善瓜蒌皮药材的质量标准，对瓜蒌皮药材的质量进行有效控制。方法：采用显微法观察瓜蒌皮粉末特征；参考中国药典2010年版附录相关方法，对瓜蒌皮的水分、总灰分、酸不溶性灰分、水溶性浸出物进行测定；采用HPLC法建立瓜蒌皮中3，29-二苯甲酰基栝楼仁三醇含量的测定方法。结果：确定瓜蒌药材显微特征；不同产地瓜蒌皮药材水分测定结果为9．65％～12．15％，总灰分为3．16％～4．99％，酸不溶性灰分为0．24％～0．46％，浸出物为32．19％～44．01％；建立HPLC法测定瓜蒌皮中3，29-二苯甲酰基栝楼仁三醇含量的方法，药材含量测定结果为0．033％～0．045％。结论：本研究建立的方法准确可靠，可用于瓜蒌皮药材的质量控制。

Objective：To establish quality standard of Trichosanthis pericarpium in order to control its quality. Methods：The characteristics of the powder of Trichosanthis pericarpium was observed by microscopic identification. The tests of water content, ash, acid-insoluble ash and water-soluble extractives of Trichosanthis periearpium were carried out according to the methods recorded in appendix of Chinese Pharmacopeia （ 2010 edition,volume 1）. The HPLC method was established to determine the content of 3,29- didenzoyl-karounitriol. Results：The microscopic characteristic of Trichosanthis pericarpium powder was identified. The results of water content, ash, acid-insoluble ash and water-soluble extractives of Trichosanthis periearpium were 9.65 % - 12.15 %, 3.16 %-4.99 %, 0.24 % - 0. 46 % and 32.19 %- 44.01% respectively. The content of 3,29-didenzoyl-karounitriol of Trichosanthis pericarpium was 0. 033 %-0. 045 % by HPLC methods. Conclusion：The established qualitative and quantitative methods in this study were accurate and reliable, which could be used for the quality control of Trichosanthis pericarpium.