摘要
目的 探讨斑点型锌指结构蛋白（SPOP）在膀胱癌中的表达及意义.方法 选择清华大学第一附属医院2012年12月至2014年3月行膀胱根治术及经尿道切除的膀胱尿路上皮癌标本32例,另选癌旁距离肿瘤组织5 cm以上的正常组织及部分前列腺手术时取出的正常膀胱黏膜20例作为正常对照,采用免疫组织化学法和蛋白质印迹法检测膀胱癌组织与正常膀胱组织中SPOP表达情况.采用蛋白免疫印迹法定性检测膀胱癌细胞株5637、T24、J82及正常膀胱细胞株SV-HUC-1中SPOP的表达水平.结果 SPOP在膀胱癌组织中表达阳性率为65.6％ （21/32）,低于正常膀胱组织[100％ （20/20）],膀胱癌组织中SPOP表达水平明显低于正常膀胱组织（0.21 ±0.08比0.73 ±0.06,P＜0.01）.膀胱癌细胞株5637、T24、J82中SPOP表达水平均明显低于正常膀胱细胞株SV-HUC-1（0.43 ±0.05、0.14 ±0.06、0.03±0.01比0.72±0.14,P＜0.01）.结论 SPOP可能是一种膀胱癌的分子标记物,与膀胱癌的发生、发展相关.
Objective To explore the expression and significance of speckle-type POZ protein （SPOP） in bladder cell cancer.Methods Totally 32 samples of bladder carcinoma and 20 samples of normal bladder either from mucosa tissue 〉 5 cm outside of the cancer tissue or from the normal bladder tissue of prostate operation were selected.The expression of SPOP in bladder cancer and normal bladder tissues were detected by immunohistochemistry and Western blot;the expressions of SPOP in bladder cancer lines 5 637,T24,J82 and normal bladder cell line SV-HUC-1 were also detected by Western blot.Results The positive rate of SPOP expression in bladder cancer was 65.6% （21/32）,lower than that in normal bladder tissue 100% （20/20）;the expression level of SPOP was significantly lower in bladder cancer tissues than that in normal bladder tissue （0.21 ±0.08 vs 0.73 ± 0.06）,and was significantly lower in bladder cancer cell lines 5 637,T24,J82 than that in normal bladder cell line SV-HUC-1 （0.43±0.05,0.14±0.06,0.03±0.01 vs0.72±0.14）（P〈0.01）.Conclusion SPOP may be a molecular bio-marker for bladder cancer,and associated with the development and progression of bladder cancer.
出处
《中国医药》
2015年第7期1071-1074,共4页
China Medicine
