促卵泡激素和血管内皮生长因子在大鼠卵巢组织冷冻自体异位移植中的保护作用

Protective Effects of FSH and VEGF on Ovarian Tissues Vitrification and Autotransplantation in Rats

目的探讨促卵泡激素(FSH)和血管内皮生长因子(VEGF)在大鼠卵巢组织冷冻自体异位移植后对卵泡内分泌功能的影响。方法 Wistar大鼠30只随机分为A、B、C组,每组10只,A组为新鲜卵巢组织移植,B组和C组卵巢组织均直接覆盖玻璃化冷冻保存2周复苏后移植,但C组在移植前2 d肌内注射FSH和VEGF,连续用药5 d。移植6周后计算卵泡密度;检测大鼠血清中雌二醇(E2)水平;卵巢组织增殖细胞核抗原(PCNA)的表达情况,并测定子宫重量。结果 A、B、C组移植后卵巢组织存活率比较差异无统计学意义(P>0.05)。A、C组卵泡密度、血清E2水平、PCNA阳性率和子宫重量均高于B组(P<0.05),A组和C组比较差异无统计学意义(P>0.05)。结论大鼠卵巢组织冷冻复苏自体异位移植前注射FSH和VEGF可以有效提高卵泡存活率,有利于恢复卵巢内分泌功能。

Objective To investigate the effects of follicle stimulating hormone（FSH） and vascular endothelial growth factor（ VEGF） on follicular endocrinic function of ovarian tissues after vitrification and autologous heterotopic transplantation. Methods A total of 30 female Wistar rats were randomly divided into group A,B and C（ n = 10 for each group）. Group A was transplanted with fresh tissues; group B and C were transplanted with resurgent tissues after cryopreserved for 2 weeks by direct cover vitrification（ DCV） method,but group C received FSH and VEGF with intramuscular injection 2 d before the transplantation for consecutive 5 d. After heterotopic auto transplantation for 6 weeks,values of follicular density were calculated; serum estradiol（ E2） levels were detected; expressions of proliferating cell nuclear antigen（ PCNA） were detected,and the values of uterine weight were measured. Results There were no statistically significance differences in survival rates of ovarian tissues among the 3 groups（ P〉0. 05）. The values of follicular density,serum E2 level,positive rate of PCNA and uterine weight in group A and C were higher than those in group B（P〈0. 05）,but there were no statistically significant differences in the values between group A and C（P〉0. 05）.Conclusion VEGF and FSH of intramuscular injection before the transplantation for ovarian tissues after vitrification and resuscitation can effectively increase the survival rate of ovarian tissues and improve the recovery of follicular endocrinic function.