半夏泻心汤对MNNG诱导大鼠前胃鳞癌的防治作用

Preventive and therapeutic effects of Banxia Xiexin Decoction on rats with forestomach squamous carcinoma induced by MNNG

目的观察半夏泻心汤对N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N'-nitro-N-nitrosoguanidine,MNNG诱导大鼠前胃鳞癌的防治作用。方法将140只雄性SD大鼠随机分为3组,空白组20只、模型组100只、模型中药组20只,采用MNNG联合多因素造模。每4周抽检模型组大鼠4只,观察前胃黏膜组织学变化,确定造模是否成功。造模成功后抽检各组大鼠,并将剩余模型组39只大鼠随机分为4组,模型对照组9只,半夏泻心汤高、中、低剂量组各10只,分别给予0.9%生理盐水、2.29、1.375、0.6875 g/m L的半夏泻心汤灌胃,10 m L/kg,1次/天,连续12周。40周末,处死全部大鼠,观察前胃黏膜组织学变化。结果 28周末前胃鳞状上皮增生、异型增生、鳞癌发生率,空白组为5%、5%、0,模型组为100%、83.3%、50%,模型中药组为0、0、0。40周末前胃鳞状上皮增生、异型增生、鳞癌发生率,模型对照组为100%、100%、77.8%,高剂量组为100%、85.7%、57.1%,中剂量组为100%、44.5%、22.3%,低剂量组为100%、77.8%、66.7%。结论半夏泻心汤对MNNG多因素造模法诱发大鼠前胃鳞癌的发生具有一定的防治作用。

Objective To observe the preventive and therapeutic effects of Banxia Xiexin Decoction on rats with forestomach squamous carcinoma induced by MNNG. Methods 140 SD rats were randomly divided into 3 groups： control group（ 20 rats）,model group（ 100 rats）,and model and traditional Chinese medicine（ TCM） group（ 20 rats）. The rat models were established with rats in all the groups except control group by using combination MNNG and multiple factors methods. 4 rats were randomly tested by observing on the histological changes of forestomach mucosa every 4 weeks,in order to determine whether the rat models establishing was successful. After rat models establishment,there were 39 rats left in model group,and the rats were randomly divided into 4 groups： model control group（ 9 rats,received gavage administration with normal saline）,high,middle and low dosage of Banxia Xiexin Decoction group（ with 10 rats in each group,received gavage administration with Banxia Xiexin Decoction with dosage of 2. 29 g / m L,1. 375 g / m L,and 0. 6875 g / m L）. The volume of gavage administration was10 m L /（ kg·d）,and the treatment lasted for 12 weeks. At the end of the 40 th week,all the rats were sacrificed,in order to observe the histological changes of forestomach mucosa. Results At the end ofthe 28 th week,the incidence rate of squamous epithelium hyperplasia,atypical hyperplasia and squamous carcinoma in forestomach was 5%,5% and 0 respectively of rats in control group,model group was100%,83. 3% and 50% respectively,while model and TCM group was 0,0 and 0 respectively. At the end of the 40 th week,the incidence rate of squamous epithelium hyperplasia,atypical hyperplasia and squamous carcinoma in forestomach of rats in model control group was 100%,100% and 77. 8% respectively,and these of rats in high dosage of Banxia Xiexin group was 100%,85. 7% and 57. 1%,middle dosage of Banxia Xiexin group was 100%,44. 5% and 22. 3%,and low dosage of Banxia Xiexin group was 100%,77. 8% and 66. 7%. Conclusion There was a certain preventive and therapeutic effects of Banxia Xiexin decoction on rats with forestomach squamous carcinoma induced by MNNG.