健心颗粒对慢性心衰大鼠心室肌toll样受体4、肿瘤坏死因子-α蛋白表达的影响

Effects of Jianxin Granule on the TLR4 and TNF-α of ventricular muscle in rats with chronic heart failure

目的探讨健心颗粒对慢性心衰大鼠心室肌toll样受体(toll-like receptors,TLR)4、肿瘤坏死因子(tumor necrosis factor,TNF)-α蛋白表达的影响。方法将15周龄的自发性高血压大鼠120只随机分为培哚普利组、健心颗粒低剂量组、健心颗粒中剂量组、健心颗粒高剂量组、阴性对照组,每组24只。前四组分别予培哚普利0.36 mg/(kg·d),健心颗粒1.6 g/(kg·d)、3.2 g/(kg·d)、6.1 g/(kg·d),以2 m L生理盐水溶解,阴性对照组予生理盐水2 m L,灌胃给药,每天2次。实验后每7天各组分别随机处死6只大鼠,采用免疫印迹检测上述各点心室肌组织TLR4蛋白表达,免疫组织化学显色检测上述各点心室肌组织TNF-а蛋白表达。结果第7天,对照组、健心颗粒低、中、高剂量组间比较,大鼠心室肌TLR4、TNF-α蛋白表达无显著差异(P>0.05),但培哚普利组明显下降(P<0.05);第14天、21天,与对照组比较,其他各组大鼠心室肌TLR4、TNF-α蛋白表达均显著下降(P>0.05),但培哚普利组较其他组下降更明显(P<0.05);第28天,与对照组比较,其他各组大鼠心室肌TLR4、TNF-α蛋白表达均显著下降(P>0.05),但培哚普利组、健心颗粒中、高剂量组明显低于低剂量组(P<0.05)。结论健心颗粒具有显著抗炎作用。

Objective To investigate the effect of the Jianxin Granule（ JG） in the expression of TLR4（ toll-like receptors 4） and TNF-α on ventricular muscle in rats with chronic heart failure. Methods rats（ SHR-sp） 120 rats,which are 15-week-old with spontaneous hypertension,were randomly divided into five groups： perindopril group 0. 36 mg /（ kg·d）,low dose JG group 1. 6 g /（ kg·d）,median dose JG group 3. 2 g /（ kg·d）,high dose JG group 6. 1 g /（ kg·d）,and negative control group（ with saline 2ml）. There are 24 rats for each group. The drugs,dissolved in 2 ml saline water,were administrated by ig twice a day. During the experiment（ 28 days）,6 rats in each group were randomly executed every 7 days.Meanwhile the expression of TLR was determined by Western blot and pro-TNF-α by immunohistochemical staining. Result On the seventh day,there were no significant difference（ P〉0. 05） of the expression of TLR4 and TNF-α in ventricular muscle among the control group,low,medium and high dose groups,but the perindopril group decreased obviously（ P〈0. 05）. On the fourteenth and the twenty-first day,compared with the control group,the expression of TLR4 and TNF-α in ventricular muscle groups significantly decreased（ P〉0. 05）,furthermore,the perindopril group decreased more significantly than the other groups. On the twenty-eighth day,the expression of TLR4 and TNF-α in ventricular muscle groups de-creased more significantly than the control group（ P〉0. 05）,however,the perindopril group,medium and high dose groups were significantly lower than those in the low dose group（ P〈0. 05）. Conclusion Jiaxin granule has a significant anti-inflammatory effect.