PLCE1表达对肝细胞癌、乳腺癌、肺癌患者预后的评价意义

Clinical Significance of Expression of Phospholipase C Epsilon-1 in Heptocellular Carcinoma,Breast Cancer and Lung Cancer

目的:探讨PLCE1与肝细胞癌、乳腺癌和肺癌的临床病理特征的关系;评价PLCE1对肝细胞癌、乳腺癌和肺癌预后评价的意义。方法:收集NCBI的肿瘤公共数据集,对手术患者肿瘤样本表达谱资料及其对应的临床信息进行病理指标的回顾性分析(卡方检验)和生存期分析(Kaplan-Meier法);利用基因集富集分析(GSEA)方法分析预测受PLCE1调控的相关基因。结果:在肝细胞癌中,高表达PLCE1的样本与高转移能力预测特征表达谱显著相关;在肺癌中,在不同性别、T分期、淋巴结浸润状态的分组中,PLCE1的表达均有显著差异;PLCE1高表达的肝细胞癌和乳腺癌患者预后更差,PLCE1低表达的肺癌患者预后更差;在不同肿瘤中,PLCE1高表达的肿瘤样本富集了不同的癌症相关基因。结论:PLCE1在不同类型肿瘤中的功能不同,其在肝癌和乳腺癌中为癌基因,在肺癌中为抑癌基因;造成PLCE1功能异质性的原因可能是PLCE1在不同类型组织中调控了不同的癌症相关基因和信号通路。

Objective： To clarify the relationship between PLCE1 expression and clinicopathological characters of hepatocellular carcinoma, breast cancer and lung caner; to evaluate the function of PLCE1 as a prognosis marker in hepatocellular carcinoma, breast cancer and lung cancer. Methods： GEO datasets were collected and tumor samples expression profile and clinical informa- tion were downloaded. The correlation between gene expression and clinicopathologic featureswas analyzed by Chi-square test. Overall survival and recurrence-free survival were analyzed by Kaplan-Meier curve. GSEA was used to predict the gene sets modulated by PLCE1. Results. PLCE1 expression was associated with predicted risk metastasis signature in hepatocellular carci- / noma （P〈0.05）; PLCE1 expression was associated with gender, T stage and lymph node status in lung cancer （P〈0.05）; Higher expression of PLCE1 indicated poor prognosis in hepatocellular carcinoma and breast cancer but better prognosis in lung cancer; GSEA indicated that PLCE1 reg- ulates different gene sets in different tumors. Conclusion. PLCE1 functions as oncogene in hepa- tocellular carcinoma and breast caner, but tumor suppressor in lung cancer; The reason for heter- ogeneity may be that PLCE1 modulates different genes and signal pathways in different tissue. Key Words