左乙拉西坦对急性癫痫小鼠海马GABA能神经元电生理的研究

Study of GABAergic neurons electrophysiological changes after levetiracetam intervention in the epileptic mouse hippocampal

目的:研究癫痫小鼠海马CA1区γ-氨基丁酸(GABA)能神经元电生理变化及使用左乙拉西坦(levetiracetam,Lev)干预治疗后的变化。方法:将FVB-Tg小鼠随机分为正常组、模型组、左乙拉西坦干预组。左乙拉西坦采用灌胃给药。在造模成功后直接断头取脑,制作脑片,在人工脑脊液中孵育后,利用膜片钳技术,进行全细胞记录。结果:与正常组相比,模型组动作电位的绝对不应期延长(P<0.05),电位能障(Vts-Vr)值上升(P<0.05),动作电位间距延长(P<0.05),差异有统计学意义。与模型组相比,左乙拉西坦干预组动作电位的绝对不应期缩短(P<0.01),电位能障(Vts-Vr)值下降(P<0.01),动作电位间距缩短(P<0.01),差异有显著统计学意义。结论:左乙拉西坦组干预后癫痫小鼠海马CA1区GABA能神经元内在特性和编码能力增强,GABA能神经元功能增加可能在癫痫的治疗中起重要作用。

AIM： To study electrophysiological changes of GABAergic neurons of epileptic mouse in CA1 region of hippocampal and changes after levetiracetam intervention. METHODS： VB-Tg small mice were randomly divided into control group,model group,and levetiracetam group. Levetiracetam was intragastrically administered to mice in the levetiracetam group. After successfully establishing the mode. The tissue blocks of hippocampus were quickly isolated. Slices were held in oxygenated artificial cerebrospinal fluid. Sequential spike patterns（ inter-spike intervals） and intrinsic properties（ARP;Vts;Vr） of GABAergic neurons were recorded by whole-cell clamp and were inputted into p Clamp 9 for data acquisition. RESULTS： Compared with control group,absolute refractory period prolonged and threshold potentials increased and inter-spike interval（ ISI） extended（ P〈0. 05） in model group,the difference was statistically significant.Compared with model group, absolute orefractory periods obviously shortened（ P〈0. 01） and threshold potentials significantly decreased（ P〈0. 01） and and inter-spike interval（ ISI） shortened（ P〈0. 01） in levetiracetam group. after levetiracetam intervention, the difference was statistically significant.CONCLUSION： After levetiracetam intervention,the spike encoding and intrinsic properties of GABAergic neurons were raised. The functional enhancement of GABAergic neurons may play an important role in epileptic control.