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猪瘟病毒衣壳蛋白与宿主细胞核仁素蛋白相互作用 被引量：2

Characterization of interaction between the capsid protein of classical swine fever virus and cellular nucleolin
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摘要 为鉴定猪瘟病毒(CSFV)衣壳蛋白(C)与宿主细胞核仁素(NCL)蛋白的相互作用,本研究将重组质粒p EGFP-CSFV-C转染至猪睾丸细胞(ST)中,采用GST pull-down和免疫共沉淀方法沉淀ST细胞中的NCL蛋白,通过共聚焦显微镜分析C蛋白和NCL蛋白的共定位情况。结果表明,CSFV C蛋白与NCL蛋白在核仁中存在共定位现象,并且在体外和转染细胞中均存在相互作用。NCL蛋白被抑制后,CSFV的复制能力下降。本研究为进一步分析C蛋白核转运机制以及在CSFV复制过程中的功能提供了实验依据。 To identify the interaction between classical swine fever virus （CSFV） capsid （C） protein and cellular nucleolin （NCL） in cells, the methods of GST pull-down and Co-IP were used precipitate the NCL protein of swine testicle （ST） cells, respectively. The results showed that cellular NCL interacts with C protein of CSFV in vitro and in transfected cells. Moreover, NCL knock down impair CSFV replication. These results provided the basis for further function studies of C transport and C protein in CSFV replication.
作者 张鑫 时洪艳 徐佳
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室 黑龙江省动物卫生监督所
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2015年第6期430-433,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金(31101823)
关键词 猪瘟病毒 衣壳蛋白 核仁素 相互作用 classical swine fever virus capsid protein nudleolin interaction
分类号 S852.65 [农业科学—基础兽医学]
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共引文献12

同被引文献27

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中国预防兽医学报
2015年 第6期

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