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伴有癫痫的人脑胶质瘤中HMGB1/TLR4的表达研究 被引量：13

Expressions of HMGB1 and TLR4 in human glioma tissues of patients combined with epilepsy
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摘要 目的 研究高迁移率族蛋白B1 （HMGB 1）/Toll样受体4（TLR4）在胶质瘤瘤体及瘤周组织的表达情况,探讨胶质瘤相关性癫痫的发生机制. 方法 收集郑州大学第五附属医院神经外科自2011年6月至2014年6月手术切除的95例胶质瘤标本,其中低级别胶质瘤（WHO Ⅰ～Ⅱ级）55例,高级别胶质瘤（WHOⅢ～Ⅳ）级40例.免疫组化染色、RT-PCR分别检测胶质瘤瘤体、瘤周组织HMGB1及TLR4蛋白和mRNA的表达. 结果 95例胶质瘤患者中,伴癫痫发作34例（35.79％）.低级别胶质瘤患者的癫痫发生率（49.09％）高于高级别胶质瘤患者（17.50％）,差异有统计学意义（x2=10.057,P=0.002）;伴发癫痫胶质瘤瘤周组织中HMGB1、TLR4蛋白表达阳性率和mRNA表达均高于不伴癫痫胶质瘤瘤周组织,差异有统计学意义（P＜0.05）;伴、不伴癫痫胶质瘤瘤体组织HMGB1、TLR4蛋白和mRNA的表达比较差异均无统计学意义（P＞0.05）. 结论 瘤周组织中高表达的HMGB1及TLR4可能参与胶质瘤相关性癫痫的发生发展,有可能成为胶质瘤相关性癫痫治疗的新靶点. Objective To investigate the expression of high mobility group protein b 1 （HMGPB1） and toll-like receptors 4 （TLR4） in human glioma tissues of patients with epilepsy and explore the mechanism of glioma-associated epilepsy.Methods Ninety-five glioma specimens,collected during the resection surgery in our hospital firom June 2011 to June 2014,were used in our experiment;among them,55 were low-grade gliomas and 40 were high-grade gliomas.Immunohistochemistry and real time-PCR were used to assay the protein and mRNA expressions of HMGB1 and TLR4 in glioma tissues and peritumoral tissues,and the relation between HMGB1/TLR4 and glioma-associated epilepsy was analyzed.Results The incidence of glioma-associated epilepsy was 35.79% （34/95）,and the incidence of glioma-associated epilepsy in low-grade gliomas （49.09%） was significantly higher than that in high-grade gliomas （17.50%）（x2=10.057,P=0.002）.Immunohistochemisty and RT-PCR showed that the protein and mRNA expressions of HMGB1/TLR4 in the peritumoral tissues with glioma-associated epilepsy were significantly higher than those in the peritumoral tissues without glioma-associated epilepsy （P〈0.05）,while no significant difference was noted between glioma tissues with and without epilepsy （P〉0.05）.Conclusion Over-expression of HMGB1 and TLR4 in peritumoral tissues of glioma patients may be related with the development of glioma-associated epilepsy,which may be a new target in glioma therapy.
作者 周少龙 王新军 付旭东 王建业 寿记新
机构地区 郑州大学第五附属医院神经外科
出处 《中华神经医学杂志》 CAS CSCD 北大核心 2015年第6期563-566,共4页 Chinese Journal of Neuromedicine
基金 河南省教育厅科学技术研究重点项目（14A320078）
关键词 癫痫 神经胶质瘤 高迁移率族蛋白B1 TOLL样受体4 Epilepsy Glioma HMGB1 TLR4
分类号 R739.41 [医药卫生—肿瘤] R742.1 [医药卫生—神经病学与精神病学]
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参考文献15

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共引文献18

同被引文献96

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引证文献13

二级引证文献62

中华神经医学杂志
2015年 第6期

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