摘要
目的观察山奈酚预处理对大鼠心肌缺血再灌注损伤的影响。方法应用Langendorff灌流装置构建大鼠心脏缺血再灌注模型,120只雄性Sprague-Dawley(SD)大鼠随机分为3组,对照组(n=40),单纯缺血再灌注组(n=40)和山奈酚处理组(n=40)。心功能指标从Langendorff灌流装置上获取;采用ELISA法检测CKMB(Creatine Kinase Isoenzyme)及SOD(superoxide dismutase)含量;采用TUNEL法检测心肌细胞凋亡。结果山奈酚预处理能显著提高大鼠心脏缺血再灌注后心功能的恢复及心肌组织内SOD的含量,同时显著降低灌流液中CK-MB的含量,减少再灌注后心肌细胞凋亡。结论山奈酚预处理对缺血再灌注后大鼠心脏具有明显的保护作用。
Objective To evaluate the effect of kaempferol against myocardial ischemia-reperfusion injury in rats.Methods Application of Langendorff simulated the model of ischemia-reperfusion.One hundred and twenty male SD rats were randomly divided into 3 groups,group A was the control group（n=40）;group B was the ischemia-reperfu-sion group（n=40）;group C was the kaempferol-treated group（n=40）.Myocardial function were recorded at the screen of Langendorff.The levels of CK-MB and SOD were determined by enzyme linked immunosorbent assay（ ELISA） ,Cardio-myocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling（TUNEL）.Results Pretreatment with kaempferol significantly improved the recovery of heart function as well as increased the levels of SOD.However, pretreatment with kaempferol reduced TUNEL-positive cell rate as well as decreased the levels of CK-MB.Conclusion Pretreatment with kaempferol provides cardioprotection in rats with I/R.
出处
《潍坊医学院学报》
2015年第3期168-170,179,共4页
Acta Academiae Medicinae Weifang
关键词
山奈酚
心脏缺血再灌注
抗氧化
抗凋亡
Kaempferol
Ischemia/reperfusion
Antioxidant
Antiapoptotic
