三七制剂对血管性痴呆大鼠海马区COX-2表达的影响

Effects of the Panax Notoginsen Preparation on Expression of COX-2 in the Hippocsampus of Rats with Vascular Dementia

目的观察三七制剂的干预对血管性痴呆(VD)大鼠行为学改变及海马区环氧化酶-2(COX-2)表达的影响。探讨三七制剂对VD大鼠发挥脑保护作用可能的相关机制。方法建立血管性痴呆大鼠模型,随机将120只雄性Wistar大鼠分为:假手术3周、6周组、模型3周、6周组;三七制剂治疗3周、6周组。运用Morris水迷宫实验评价认知行为学改变,免疫组织化学法观测大鼠海马区COX-2的表达。结果三七制剂的干预可以提高血管性痴呆大鼠的学习记忆力和反应能力;三七制剂治疗组和模型组COX-2表达均较假手术组明显增高(P<0.01),但三七制剂治疗组COX-2表达明显低于模型组(P<0.01)。结论三七制剂的干预可以使血管性痴呆大鼠的行为学症状得到明显改善,其机制是通过对大鼠脑组织COX-2表达的抑制而发挥其脑保护作用。

Objective To observe the Panax Notoginseng preparations of intervention on vascular dementia （ VD） and the change of behavior in rats hippocampus cyclooxygenase-2（ COX-2） expression of its effect.To explore the probable mechanism of the Panax Notoginseng agents effect on cerebral protection of rats with VD.Methods The model of VD rats were set up and 120 Wistar rats were randomly divided into sham operation group with 3W,6W,model group with 3W,6W or Panax Notoginseng preparation for treating group with 3W,6W.Evaluation of the cognitive behavioral changes was made by Morris water maze test,observation of COX-2 expression in rat hippocampus was tested with immu-nohistochemical.Results The Panax Notoginseng preparations of the intervention improved VD rats＆#39;ability to learn, memorize and react.Expression of Panax Notoginseng preparations in treatment group and model group COX-2 were sig-nificantly higher than those in the sham operation group（P〈0.01）,but the Panax Notoginseng agents for the treatment group with COX-2 expression was significantly lower than that of model group（ P〈0.01） .Conclusion The Panax Noto-ginseng preparations can obviously improve praxiology symptom of rats with VD, and the development of its protective effect on brain probably by means of inhibiting expression of COX-2 in the brain.