血清胱抑素C在急性肾损伤早期的诊断价值分析

Value of Serum Cystatin C Level in the Early Diagnosis of Sepsis-Induced Acute Kidney Injury

目的探讨血清胱抑素C(Cys C)在大鼠脓毒症致急性肾损伤早期诊断中的价值。方法健康雄性SD大鼠60只,随机分为健康对照组、假手术组、脓毒症组。脓毒症组采用盲肠结扎穿孔术(CLP)制作脓毒症模型,各组于术后3,6,12,24h分别留取血液,检测血肌酐(CREA)、尿素(UREA)及Cys C,术后取肾组织行病理检查。结果与健康对照组相比,假手术组及脓毒症组术后24h内血清UREA,CREA,Cys C水平均未发生显著性变化(P>0.05)。健康对照组与假手术组肾组织基本正常,而脓毒症组肾组织病理改变明显。结论在急性肾损伤发生后24h内,Cys C不是有价值的标志物。

Objective To determine the value of serum cystatin C level in the early diagnosis of sepsis-in-duced acute kidney injury in rats.Methods Sixty healthy male SD rats were randomly assigned to three groups：sepsis group,sham operation group and controlled group.The sepsis models were reproduced by cecal ligation and puncture （CLP） method.Blood and urine samples of all groups were collected 3,6,12 and 24 hours after operation to test CREA, UREA and Cys C,pathology of the kidney were examined after operation,too.Results Compared with controlled group, the level of serum UREA,CREA and Cys C of the sham operation group and sepsis group did not change significantly during 24 hours（P〉0.05）.The renal tissue of controlled group and sham operation group were basically normal,and re-nal pathology of sepsis group changed obviously.Conclusion Serum cystatin C is not a valuable maker of sepsis-in-duced acute kidney injury in 24h.