新型抗菌仿生硅化胶原支架的制备

Development of a novel antibacterial intrafibrillar-silicified collagen scaffold

目的:制备抗菌仿生硅化胶原材料。方法:使用浊度分析确定稳定的正硅酸矿化液中葡萄糖酸氯己定的最小有效浓度,用氯己定稳定的硅酸前体液,对胶原海绵进行仿生硅化处理,并通过傅里叶红外光谱仪分析及透射电子显微镜(TEM)观察确定硅化效果。结果:20 g/L的葡萄糖酸氯己定可使正硅酸稳定3 d而不发生凝胶化,以此为矿化液可使经聚丙烯氯化铵处理的胶原支架发生纤维内硅化。TEM观察结果显示,纤维内矿物质充实了胶原纤维内部空隙,傅里叶红外光谱分析中的C=C伸缩振动峰表明,在仿生硅化胶原引入了葡萄糖酸氯己定,形成抗菌仿生纤维内硅化胶原。结论:以胶原为模板、聚丙烯氯化铵为催化剂、氯己定稳定的硅酸前体液为矿化基材,成功制备了新型抗菌仿生硅化胶原支架。

AIM： To develop a novel antibacterial intrafibrillar-silicified collagen scaffold. METHODS：Silicic acid solution was prepared by hydrolyzing tetraethyl orthosilicate. A chlorhexidine stabilized silicic acid was pre-pared and used as silicifying medium. Gelling time test was used to determine the minimum concentration of gluconic acid chlorhexidine that was able to stabilize silicic acid for 72 hours. The reconstituted type I collagen sponge disc was pretreated with 6. 67 × 10 -4 M poly allylamine hydrochloride for 4 h and then placed in the silicifying medium for 4 d to produce silicified collagen scaffold. Attenuated total reflection-fourier transform infrared spectroscopy （ ATR-FTIR） and transmission electron microscopy （ TEM） were used to characterize the antibacterial intrafibrillar-silicified collagen scaffold. RESULTS：2. 0% gluconic acid chlorhexidine could stabilize the silicic acid for 3 d without gelation and was used as silicifying medium. With this method, silicic acid could infiltrate into the inside space of the type I collagen and then condense into orderly deposited silicon dioxideinside collagen fibrils, resulting in intrafibrillar-silicified colla-gen scaffold doped with chlorhexidine. FTIR confirmed the incorporation of silica and chlorhexidine in the scaffold. CONCLUSION：A novel antibacterial intrafibrillar-silicified collagen scaffold is developed with reconstituted type I collagen sponge pretreated by poly allylamine hydrochloride and silicic acid stabilized by gluconic acid chlorhexidine.