红芪多糖对人牙周膜细胞增殖及分泌IL-1β、IL-6的影响

Effect of radix hedysari polysaccharide on the proliferation and expression of IL-1β,IL-6 in human periodontal ligament cells

目的:研究红芪多糖(HPS)对人牙周膜细胞(h PDLCs)增殖及分泌IL-1β、IL-6的影响。方法:采用改良组织块酶消化法培养人牙周膜细胞,经免疫组化SP法进行鉴定,MTT法检测细胞增殖情况,荧光实时定量PCR和酶联免疫法检测红芪多糖对LPS作用的h PDLCs分泌IL-1β、IL-6的影响。结果:10μg/m L HPS能够促进人牙周膜细胞增殖(P<0.05);10μg/m L LPS可显著刺激人牙周膜细胞分泌IL-1β、IL-6(P<0.01),而给予10μg/m L HPS能抑制LPS刺激人牙周膜细胞分泌IL-1β、IL-6,且与LPS组具有显著性差异(P<0.01)。结论:适宜浓度的红芪多糖能够促进体外培养的人牙周膜细胞增殖,并抑制LPS作用的人牙周膜细胞分泌IL-1β、IL-6。

Objective：The purpose of this study is to observe the effect of radix hedysari polysaccharide（HPS） on the proliferation and expression of interleukin（IL）-1β,IL-6 in human periodontal ligament cells（hPDLCs） and to investigate the effect of HPS on the periodontal inflammatory reaction. Method：Primary hPDLCs were cultured by tissue block enzymolytic method,cells were used after immunohistochemical identification（with SP method） in this experiment. 3-（4,5-Dimethylthi-azol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay were used to examine the effect of HPS on the proliferation of hPDLCs. The effect of HPS on the expression of IL-1β,IL-6 in hPDLCs treated with LPS were determined by quantitative real-time polymerase chain reaction（qRT-PCR） and enzyme-linked immunosorbent assay（ELISA）. Result：HPS at a con-centration of 10 μg/mL promoted hPDLCs proliferation.The expression of IL-1β,IL-6 was significantly increased with E. Coli LPS at 10μg/mL（P〈0.01）. After adding HPS at 10 μg/mL,the production of IL-1β,IL-6 was inhibited（P〈0.05）. Conclusion：Radix hedysari polysaccharide could stimulated the cell proliferation in vitro,inhibit the expression of IL-1β, IL-6 in human periodontal ligament cells treated with LPS. As a traditional Chinese herb,its mechanism of action of peri-odontal inflammation still needs further study.