PDGF-BB促进人牙髓间质细胞成牙本质分化的实验研究

PDGF-BB promotes human pulp stroma cells odontoblastic differentiation

目的:检测PDGF-BB是否可促进人类牙髓细胞成牙本质分化过程。方法:体外原代培养牙髓细胞,将PDGF-BB作用于人类牙髓间质细胞,检测牙髓间质细胞在矿化诱导培养基的分化。利用实时定量PCR检测分化相关指标牙本质涎磷蛋白(DSPP)及牙本质基质蛋白1(DMP1)的表达水平。利用免疫印迹技术检测PDGF-BB处理后的人牙髓间质细胞胞内AKT信号分子的活化情况。结果:PDGF-BB可明显促进人类牙髓间质细胞矿化结节的形成。同时,利用实时定量PCR检测发现巨噬细胞上清处理的牙髓间质细胞的DSPP及DMP1表达明显上调。且牙髓间质细胞内的AKT磷酸化水平明显升高。结论:PDGF-BB可促进牙髓间质细胞的成牙本质分化,可能在牙本质形成过程中发挥重要作用。

Objective：To investigate whether PDGF-BB promotes the odontoblastic differentiation of human pulp stromal cells. Method：The effects on the odontoblastic differentiation of human pulp stromal cells were performed using the mineralization medium absence of PDGF-BB. Moreover,the DSPP and DMP1 mRNA expression levels of human pulp stro-mal cells were analyzed using real-time PCR assays. And,the activation of AKT in human pulp stromal cells was measured by western blots. Result：PDGF-BB promotes the odontoblastic differentiation of the human pulp stromal cells. Real time PCR revealed that the mRNA levels of DSPP and DMP1 in human pulp stromal cells were significantly increased. The phosphor-AKT was found up-regulated in the PDGF-BB treated human pulp stromal cells. Conclusion：PDGF-BB could promote the odontoblastic differentiation and odontoblastic proteins expression of human pulp stromal cells,which might play roles in the development of dentin formation.