银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响

Effects of GBE on the Proliferation and Osteogenic Differentiation of BMSCs

目的:研究银杏叶提取物(ginkgo biloba extract,GBE)对大鼠的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖及成骨分化过程的影响。方法:将不同浓度的GBE作用于第3代BMSCs,通过CCK-8(Cell Counting Kit-8)法及流式细胞仪法检测其对细胞增殖活性变化的影响,通过茜素红染色及碱性磷酸酶(Alkalinet phosphatase,ALP)法判定其对细胞成骨能力的影响。RT-PCR检测成骨相关基因骨形成蛋白2(bone morphogenetic protein-2,BMP-2)、Runx2(runt-related transcription factor2)的mRNA的表达。结果:不同浓度的GBE对BMSCs的增殖无明显影响,但能使细胞处于S期,显著促进ALP的表达,在浓度为150mg/L时表达最多。其成骨相关基因BMP-2、Runx2的mRNA表达也相对增高。结论:银杏叶提取物对骨髓间充质干细胞的成骨分化具有积极意义。

Objective：To study the effects of GBE on proliferation and osteogenic differentiation process of rat BMSCs.Methods：By applying different concentrations of GBE on the third-generation of BMSCs,we evaluate its influence on the cell proliferation activity through CCK-8assay and flow-cytometry analysis.Then we estimated the effect of osteogenic ability of GBE by alizarin red S and alkalinet phosphatase method.RT-PCR was taken to test the expression of the osteogenic specific genes（BMP-2,Runx2）mRNA.Results：There were no statistically significant differences between different concentrations of ginkgo biloba extract in the proliferation on bone marrow mesenchymal stem cells.However,GBE could make the cells in S phase of the cell cycles.GBE could significantly promote the expression of alkaline phosphatase,especially when the concentration was 150 mg/L.The osteogenic specific genes（BMP-2,Runx2）mRNA expression were also relatively higher than control group.Conclusion：Ginkgo biloba extract has a significantly positive effect on the osteogenic differentiation of the BMSCs.