EB病毒微RNAs在结肠癌中的差异表达及其靶基因的功能预测

Differential expression analysis of EB virus microRNAs and the functions of their candidate targets in colorectal cancer

目的筛选结肠癌样本中差异表达的EB病毒微RNAs（microRNAs），并对其靶基因进行功能预测分析。方法采用Exiqon LNATM microRNA芯片分析结肠癌组织和癌旁组织之间差异表达的内源microRNAs和EB病毒microRNAs。以人类mRNA为靶标，采用3种方法预测EB病毒microRNAs的靶基因，并通过GeneOntology分析与分析预测靶基因的生物学功能。结果芯片分析得到3个相对于癌旁组织和结肠癌组织表达显著下调的EB病毒microRNAs，生物信息学分析预测其靶基因有329个，其中48个（14．6％）靶基因参与细胞膜组成，86个（26．1％）靶基因在离子结合方面发挥功能，并影响代谢通路、钙离子信号转导、Wnt信号、T细胞受体等多个信号通路。结论筛选得到3个结肠癌异常表达的EB病毒microRNAs，其靶基因参与信号传导和物质代谢路径，可能影响结肠癌发生发展。

Objective To identify differentially expressed EB virus （EBV） microRNAs in colorectal cancer and analyze the potential functions of their candidate target genes. Methods MicroRNAs expression was evaluated by Exiqon LNATM microRNA chipin colorectal tissues and adjacent non-tumor tissues. EBV microRNAs targeting genes were predicted through three methods. The human mRNA database was used as reference, and analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomesanalysis. Results Three EBV microRNAs of were identified by chip analysis which were obviously down regulated, compared with from the colorectal cancer tissue and para-carcinoma tissue. There were 329 targets predicted by biological information analysis. Among them, a total of 48 targets （14.6%） participated in cell membrane and 86 targets （26.1%） played a role in ion binding and impacted many signaling pathways, such as metabolic pathways, calcium signaling pathway, Wnt signaling pathway and T cell receptor. Conclusions A total of 3 differentially expressed EBV microRNAs regulate multiple human genes in colorectal cancer, and these genes are closely related to occurrence and progression of the disease by participating in metabolic pathways and signaling pathways.