Fasudil 对实验性自身免疫性脑脊髓炎小鼠模型巨噬细胞的免疫调节作用研究

The immunoregulatory effect of Rho kinase inhibitor Fasudil on macrophages in a mouse model of ;experimental autoimmune encephalomyelitis

目的：探讨Fasudil（法舒地尔）介导的细胞治疗对实验性自身免疫性脑脊髓炎（ exper-imental autoimmune encephalomyelitis， EAE）巨噬细胞的免疫调节作用。方法雌性C57BL／6小鼠，采用髓鞘少突胶质细胞糖蛋白35-55（MOG35-55）诱导建立主动免疫EAE模型，免疫后第9天制备脾脏单个核细胞（ MNCs），在添加／或无Fasudil条件下体外培养。72 h后收集上述细胞，应用流式细胞术检测巨噬细胞亚群的变化，Griess法检测NO含量，ELISA法检测Arg-1水平；并以5×10^7个细胞／只小鼠腹腔注射诱导建立被动转移EAE模型，分为PBS-MNCs组和Fasudil-MNCs组，观察各组小鼠临床评分和体重变化。结果主动免疫第9天EAE小鼠脾脏MNCs可诱导被动转移EAE模型的建立，而体外Fasudil处理72 h的MNCs可影响EAE的发生、发展。体外实验显示，Fasudil可部分逆转M1表型至M2表型，即M1型标志CD16／32、iNOS、IL-12降低，而M2型标志CD206、IL-10、CD23升高。 Fasudil还可抑制NO产生，促进Arg-1分泌。结论 Fasudil通过转化炎性M1型巨噬细胞为抗炎的M2型巨噬细胞影响EAE的发生、发展。

Objective To investigate the immunoregulatory effect of Fasudil-modified macrophages on cell transferred experimental autoimmune encephalomyelitis （ EAE） in a mouse model.Methods Fe-male C57BL/6 mice were immunized with MOG35-55 to establish the model of EAE.The encephalomyelitic mononuclear cells （ MNCs） were isolated from spleen of mice with EAE on day 9 after immunization and treated with or without Fasudil for 72 h in vitro.Several assays including the flow cytometry analysis, Griess reaction and ELISA were performed to analyze the M1 and M2 phenotypes of macrophages, the production of NO and the levels of cytokines, respectively.The cultured MNCs （5×10^7 cells） were resuspended in 500μl of PBS and transferred into na？ve C57BL/6 recipients via intraperitoneal injection.Two groups including the PBS-MNCs group and the Fasudil-MNCs group were set up.The body weights and clinical scores of the mice in each group were recorded in every other days after the induction of EAE in the recipients.Results The Fasudil treated MNCs affected the induction of EAE in adoptive cell transferred mice.The expression of CD16/32, iNOS and IL-12 on F4/80-macrophages were decreased, while the expression of CD206, CD23 and IL-10 on F4/80-macrophages were increased upon the treatment of Fasudil, indicating that Fasudil im-proved the differentiation of macrophages from M1 to M2 phenotypes.Moreover, Fasudil inhibited the pro-duction of NO and enhanced the expression of Arginase-1.Conclusion Fasudil ameliorated the clinical se-verity of EAE in mice by promoting the transformation of macrophages from M1 to M2 phenotype.