Apaf-1和 AEG-1在结肠癌中的表达及意义

Expression and clinical significance of Apaf-1 and AEG-1 in colonic carcinoma

目的：检测结肠癌组织中凋亡蛋白酶活化因子-1（ Apaf-1）和星形细胞上调基因-1（AEG-1）的表达情况，并探讨其与临床病理特征的关系。方法采用免疫组织化学法检测63例结肠癌和30例癌旁正常组织中 Apaf-1和 AEG-1的蛋白表达，分析其与结肠癌临床特征的关系。结果结肠癌组织中 Apaf-1和 AEG-1表达阳性率分别为23.81％（15/63）和68.25％（43/63），正常结肠组织中表达阳性率分别为76.67％（23/30）和26.67％（8/30）。AEG-1在癌组织中表达高于癌旁正常组织（χ^2＝14.192，P ＝0.000），而 Apaf-1的表达明显低于癌旁正常组织（χ^2＝23.497，P ＝0.000），两者表达呈负相关（r ＝-0.339，P ＝0.007）。AEG-1和 Apaf-1的表达与结肠癌组织分化程度（χ^2＝4.643，P ＝0.031；χ^2＝12.034，P ＝0.001）和临床分期（χ^2＝6.628，P ＝0.010；χ^2＝8.246，P ＝0.004）相关，而与患者年龄（χ^2＝1.462，P ＝0.227；χ^2＝2.401，P ＝0.121）及肿瘤大小（χ^2＝0.333，P ＝0.564；χ^2＝0.590，P ＝0.442）无相关性。结论 AEG-1在结肠癌中表达明显上调，而 Apaf-1表达下调，二者呈负相关。Apaf-1和AEG-1可能与结肠癌的发生、发展密切相关，联合检测二者可能对结肠癌的预后判断更具价值。

Objective To study the expressions of apoptotic protease activating factor-1（Apaf-1）and astrocyte elevated gene-1（AEG-1）in colonic carcinoma,and to explore their correlations with the clinical path-ological features. Methods The expressions of Apaf-1 and AEG-1 were detected in 63 colonic carcinoma samples and 30 normal colonic mucosa adjacent to tumor nest by immunohistochemical method,and their correlations with clinical features of colonic carcinoma were analyzed. Results The positive expressions of Apaf-1 and AEG-1 in colonic carcinoma were 23. 81%（15 / 63）and 68. 25%（43 / 63）,respectively. The positive expressions of Apaf-1 and AEG-1 in normal colonic mucosa were 76. 67%（23 / 30）and 26. 67%（8 / 30）,respec-tively. The positive expression rate of AEG-1 was significantly higher in colonic carcinoma than that in normal tissue（χ^2 = 14. 192,P = 0. 000）. However,the expression of Apaf-1 was signi-ficantly lower in colonic carci-noma than that in normal tissue（χ^2 = 23. 497,P = 0. 000）. The expression of Apaf-1 was negatively correlated to the expression of AEG-1（r = - 0. 339,P = 0. 007）. The expressions of AEG-1 and Apaf-1 were associated with differentiation degree（χ^2 = 4. 643,P = 0. 031;χ^2 = 12. 034,P = 0. 001）and clinical stage（χ^2 = 6. 628, P = 0. 010;χ^2 = 8. 246,P = 0. 004）,but they were not correlated with age（χ^2 = 1. 462,P = 0. 227;χ^2 =2. 401,P = 0. 121）and tumor size（χ^2 = 0. 333,P = 0. 564;χ^2 = 0. 590,P = 0. 442）. Conclusion The expression of AEG-1 is up-regulated in colonic carcinoma,but the expression of Apaf-1 is down-regulated,with a significant negative correlation. Apaf-1 and AEG-1 may be closely related to the occurrence and development of colon carcinoma. Therefore,combination detection of Apaf-1 and AEG-1 may be more valuable for the prognosis evaluation of colonic carcinoma.