石菖蒲挥发油对东莨菪碱致大鼠学习记忆障碍的影响及作用机制研究

Effects of Acorus Tatarinowii Schott volatile oil on learning and memory impairment rats induced by scopolamine and its possible mechanism

目的研究石菖蒲挥发油对东莨菪碱所致大鼠学习记忆障碍的影响及可能的作用机制。方法将40只大鼠随机分为正常对照组、模型组、多奈哌齐组(0.25 mg/kg)、石菖蒲挥发油组(12 g/kg),每组10只,每天灌胃给药1次,连续灌胃21 d。第15天起,按2.1 mg/kg的剂量腹腔注射东莨菪碱,1次/d,连续给药7 d以制备大鼠学习记忆障碍模型。通过Morris水迷宫法检测大鼠学习记忆能力,免疫组化法分析海马组织中胶质纤维酸性蛋白(GFAP)的表达,酶联免疫法检测大鼠海马组织中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果石菖蒲挥发油能显著缩短模型大鼠逃避潜伏期,增加模型大鼠穿越平台次数,显著降低模型大鼠海马组织中GFAP的表达量和MDA的含量,升高SOD的含量。结论石菖蒲挥发油可以有效改善东莨菪碱所致的学习记忆障碍模型大鼠的学习记忆能力,其作用机制可能与其下调海马区星形胶质细胞内GFAP的表达以及抗氧化作用有关。

Objective To investigate volatile oil of Acorus tatarinowii Schott on rats with learning and memory impairment induced by scopolamine and its possible mechanism. Methods 40 rats were randomly divided into normal control group, model group, aricept group （ 0.25 mg/kg） , volatile oil of Acorus tatarinowii Schott group （12 g/kg）. Volatile oil of Acorus tatarinowii Schott was given for 21 days by lavage administration, intraperitoneal injection of scopolamine （2.1 mg/kg） was given for 7 days to prepare the model rats with learning and memory disorder. Morris water maze experiment was used to test the learning and memory ability. GFAP expression in hippocampal tissue was analyzed by immunohistochemical. The content of SOD and MDA in the rats＇ hippocampus was detected by enzyme-linked immunoassay. Results The volatile oil of Acorus tatarinowii Schott significantly shortened the model rats escaping latent period, increased the frequency of crossing-over, reduced the expression of GFAP in model rats＇ hippocampal tissue, significantly reduced the content of SOD and MDA. Conclusion The volatile oil of Acorus tatarinowii Schott can improve the learning and memory ability of rats induced by scopolamine, its mechanism may be linked with the reduction of the expression of GFAP protein in hippocampus astrocytes and antopxidation.