摘要
目的建立一种脂肪乳中甘油含量测定的方法。方法甘油可以被高碘酸钠氧化生成甲醛,甲醛与乙酰丙酮反应生成的衍生物有紫外吸收,采用紫外分光光度计测定衍生物的吸光度。结果在测定的含量线性范围内,回收率为100.04%,方法精密度良好。结论该方法可以准确地测定脂肪乳中的甘油含量。
Objective To establish a new method for the determination of glycerin in fat emulsion. Method Glycerin was oxidized by sodium periodate to generate formaldehyde, which in turn reacted with acetylacetone to produce a derivative with ultraviolet absorption property, which was determined using ultraviolet spectrophotometer. Result This method showed high recovery ( 100. 04% in the linear range of content) and good precision. Conclusion This method is reliable in determining the concentration of glycerin in fat emulsion.
出处
《中华临床营养杂志》
CAS
CSCD
2015年第3期176-178,共3页
Chinese Journal of Clinical Nutrition
关键词
脂肪乳
甘油
含量测定
Fat emulsion
Glycerin
Content determination