子痫前期患者CD4^+CD25^+调节性T细胞亚群的改变及意义

Changes of regulatory T cells in peripheral blood of patients with preeclampsia

目的探讨子痫前期患者CD4^+CD25^+调节性T细胞(Treg)亚群改变及在PE免疫耐受失衡中的作用。方法选取PE患者(观察组)30例,同期正常晚期妊娠(对照组)20例。分别采集PE患者和正常晚期妊娠妇女外周血,采用流式细胞术检测Treg以及活化记性相关(CD45RO、HLA-DR)、归巢相关(CCR4、CCR6、CD103)、功能相关(ICOS、CTLA-4、Galectin 1)和生存相关(PD1和CD95)标记分子的表达。采用免疫抑制实验检测外周血中Treg的免疫抑制功能。结果观察组患者外周血CD4^+CD25^+细胞的比例[(5.87±3.34)%]低于对照组[(6.65±1.18)%],但差异无统计学意义。免疫抑制实验显示观察组患者外周血中Treg对CD4+CD25-T细胞的增值抑制作用与对照组无统计学差异。通过对Treg多个标记分子的检测进一步发现,ICOS和CCR6在观察组Treg上的表达水平显著低于对照组Treg(均P<0.01);CCR4和Galectin 1的表达水平较对照组低,而CD45RO、HLA-DR、CD103、CTLA、PD1和CD95的表达水平较对照组高,但两组间差异均无统计学意义(均P>0.05)。结论 Treg亚群比例失调可能是导致子痫前期发生的重要因素。

Objective To investigate the changes of regulatory T cel s（CD4＋CD25＋Treg） in peripheral blood of patients with preeclampsia（PE）. Methods Peripheral blood of 30 patients with PE and 20 normal gravidas with late pregnancy were en-rol ed. The expression of CD4＋CD25＋Treg and its markers related to activation （CD45RO, HLA- DR）, homing （CCR4, CCR6, CD103）, function （ICOS, CTLA- 4, Galectin 1） and survival （PD 1 and CD95） were detected using flow cytometry. Immunosup-pression function of Treg in vitro was detected by immunosuppression assay, Results The proportion of CD4＋CD25＋Treg a-mong CD4＋T cel s was 5.87±3.34%in patients with PE, lower than that in control group （6.65±1.18%, P〉0.05）. Immunosup-pression assay showed similar suppression power of Treg from the two groups. The expression of ICOS and CCR6 on Treg in patients with PE was significantly lower than that in normal pregnancy subjects （P〈0.01）. The expression of CCR4 and Galectin 1 was decreased but that of CD45RO, HLA- DR, CD103, CTLA, PD1 and CD95 was increased in patients with PE. However, the differences between the two groups were not significant. Conclusion Imbalance of Treg subsets may be an important factor leading to the occurrence of preeclampsia.