HPLC法同时测定肾炎四味胶囊中4种黄酮类成分的含量

Determination of Four Flavonoids in Shenyansiwei Capsules by HPLC

目的：建立HPLC法同时测定肾炎四味胶囊中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素等4种成分。方法：采用Agilent ZORBAX SB—C18（250mm×4．6mm，5μm）色谱柱，流动相为甲醇-0．4％磷酸溶液，梯度洗脱，流速为1．0ml·min^-1，检测波长为278nm，柱温为30℃，进样量为10μl。结果：上述色谱条件下，4种成分分离较好，黄芩苷、黄芩素、汉黄芩苷和汉黄芩素线性关系良好，线性范围分别为0．060～1．203μg（r=0．9999）、0．026～0．521μg（r=0．9999）、0．031—0．622μg（r=0．9998）、0．025—0．497μg（r=0．9996），加样回收率分别为97．9％（RSD=1．72％）、101．8％（RSD=1．86％）、102．1％（RSD=1．35％）、99．3％（RSD=1．12％）（n=9）。结论：本方法准确度高、重复性好，能同时测定肾炎四味胶囊中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素等4种成分的含量。

Objective： To establish an HPLC method for the simultaneous determination of four kinds of flavonoids （ baiealin, wogonoside, baiealein and wogonin） in Shenyansiwei ,capsules. Methods： An Agilent ZORBAX SB-C18 eolumn（250mm×4.6mm,5μm） was used, the mobile phase consisted of methanol-0.4% phosphoric acid solution with gradient elution, the flow rate was 1.0 ml ·min^-1 the detection wavelength was set at 278 nm, the column temperature was 30℃, and the injection volume was 10 μl Results： The four kinds of flavonoids were well separated and showed good linearity. The linear range of baiealin, wogonoside, baiealein and wogonin was O. 060-1. 203 μg（ r = 0 999 9 ） ,0. 026-0. 521 μg（ r = 0. 999 9 ） ,0. 031-0. 622 μg（ r = 0. 999 8 ） and 0. 025-0. 497 μg（ r = 0.999 6 ）, respectively. The average reeovery of the four kinds of flavonoids was 97.9% （ RSD = 1.72%, n = 9 ）, 101.8% （ RSD = 1.86%, n = 9）, 102.1% （RSD = 1.35%, n = 9） and 99.3% （RSD = 1.12%, n = 9 ）, respectively. Conclusion： The method is rapid, simple, reliable and aeeurate, which can be successfully used in the quantification of the four constituents in Shenyansiwei capsules.