HPLC法同时测定和肝利胆颗粒中栀子苷和黄芩苷的含量

Simultaneous Determination of Geniposide and Baicalin in Hegan Lidan Granules by HPLC

目的：建立和肝利胆颗粒中栀子苷和黄芩苷的含量测定方法。方法：采用高效液相色谱法，用Dionex C18（250mm×4．6mm，5μm）色谱柱，以甲醇为流动相A，0．5％冰醋酸溶液为流动相B，梯度洗脱，流速为1．0ml·min^-1，检测波长为239nm，柱温：30℃，进样量：10μl。结果：栀子苷和黄芩苷与其相邻杂质峰能完全分离，栀子苷在7．890—78．900μg·ml^-1的浓度范围内有良好的线性关系，r=0．9999；黄芩苷在8．020～80．200μg·ml^-1的浓度范围内有良好的线性关系，r=0．9999。栀子苷和黄芩苷的平均回收率分别为99．8％（RSD=2．1％，n=6）和99．5％（RSD=1．5％，n=6）。结论：本方法简便、准确、重复性好，可用于该药的质量控制的评价。

Objective ： To establish a method for the determination of geniposide and baicalin in Hegan Lidan granules. Methods： Dionex C18（250mm×4.6mm,5μm）column was used with the mobile phase of methanol-0.5% glacial acetic acid solution with gradient elution and the flow rate was 1.0 ml · min^- 1. The detection wavelength was 239 nm, the column temperature was 30℃ and the injection volume was 10μl. Results： Geniposide and baicalin could be completely separated from the impurities. The linear range of geniposide and baicalin was 7. 890-78. 900 μg · ml^ -1 （ r = 0. 999 9） and 8.020-80.200 μg· ml^ -1 （ r = 0. 999 9） with the average recovery of 99.8 % （ RSD = 2.1% , n = 6） and 99.5 % （RSD = 1.5 %, n = 6）, respectively . Conclusion： The methods is simple, accurate and reproducible. It can be used in the quality control of Hegan Lidan granules.