气相色谱法测定阿莫西林中甲醇残留

Determination of Residual Methanol in Amoxicillin by GC

目的：建立阿莫西林中甲醇残留量的检测方法。方法：用气相色谱法测定阿莫西林中甲醇残留量。色谱柱为安捷伦HP-1毛气管气相色谱柱（30m×0．53mm，2．65μm）；氢火焰离子化检测器（FID），温度280℃；进样口温度150℃；载气为高纯氮气；柱流速2～6ml·min^-1；顶空进样，保温温度80℃。结果：甲醇在3．29～210．48μg·ml^-1范围内线性关系良好（r=0．9999），平均回收率为98．7％（RSD=0．9％，n=9）。结论：该方法简便、准确、可靠，适用于阿莫西林中甲醇残留量的检测。

Objective ： To estoblish the detection method for residual methanol in amoxicillin. Methods： GC was applied to detect residual methanol in amoxicillin. The chromatographic column was an Agilent HF-1 capillary column （30m×0.53mm,2.65μm）. The flame ionization detector （FID） was used with the temperature of 280℃, the injection port temperature was 150℃, the cartier gas was nitrogen with high purity, the flow rate was 2-6 ml · min^-1 , the injection mode was headspace injection, and the holding temperature was 80℃. Results： There was a good linear relationship with the concentration of methanol within the range of 3.29-210.48 μg · ml^-1 （r = 0.999 9）, and the recovery was 98.7% （RSD = 0.9% , n = 9 ）. Conclusion： The method is simple, accurate and reliable, and suitable for the detection of residual methanol in amoxicillin.