SOD1-G93A小鼠中枢神经系统SIRT1的变化及白藜芦醇对其的影响

SITR1 expression and the effects of resveratral on SIRT1 in the motor cortex and lumber spinal cord of SOD1-G93A mice

目的观察SOD1-G93A小鼠运动皮质和腰髓中SIRT1的变化以及白藜芦醇对SIRT1的影响。方法利用SOD1-G93A小鼠及其野生型小鼠作为实验对象,分别检测随着疾病进展小鼠神经系统中SIRT1表达的动态变化及给予白藜芦醇后SIRT1的变化。结果随着ALS疾病进展,症状早期及终末期SIRT1表达增多,而给予白藜芦醇后,SIRT1表达无明显变化,给予溶剂后SIRT1表达增多。结论随着疾病进展,SOD1-G9A小鼠运动皮质与腰髓中SIRT1的表达逐渐增多;白藜芦醇对SIRT1未起到激活作用,白藜芦醇在SOD1-G93A小鼠模型中有无有益作用尚不能定论。

Objective To observe the changes in motor cortex and lumber spinal cord of the SOD1G93 A mice and the influence of resveratrol on SIRT1. Method Use SOD1G93 A mice and its wild type as the experimental objects. As the development of the disease,inspect the change of SIRT1 and the influence of resveratrol on SIRT1 in the micenerve system respectively. Result The expression of SIRT1 increased in the early and late stages of the diease,when given resveratrol the expression of SIRT1 didn＇t change much,but increased after given solvent. Conclusion As the development of the disease,the expression of SIRT1 increased gradually in motor cortex and lumber spinal cord of SOD1G93 A mice,Resveratrol doesn＇t activate SIRT1,we can＇t make the conclusion whether resveratrol is beneficial or not in the mode of SOD1G93 A mice.